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Alberta NINT/19 June 2008
From 2008.igem.org
(Difference between revisions)
(New page: lab work (SD): Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. Set up for sequencing.) |
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Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. | Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol. | ||
Set up for sequencing. | Set up for sequencing. | ||
| + | |||
| + | lab work (JD): | ||
| + | Counted and recorded number of colonies on newly plated K102007 plates from yesterday | ||
| + | K102007 with the newly recut TA0: 128 | ||
| + | K102007 with heated old TA0: 162 | ||
| + | Negative control: 176 | ||
| + | hence ligation didn't work AGAIN, tomorrow we will try something different and redo ligation on Monday | ||
| + | Mini-prep of 7 O/N colonies of K102003.1-K102003.7 | ||
| + | Set up for sequencing | ||
Revision as of 19:57, 19 June 2008
lab work (SD):
Isolated DNA (K102002) from LB culture tubes from 18/06/08 using QIAprep spin miniprep protocol.
Set up for sequencing.
lab work (JD):
Counted and recorded number of colonies on newly plated K102007 plates from yesterday
K102007 with the newly recut TA0: 128
K102007 with heated old TA0: 162
Negative control: 176
hence ligation didn't work AGAIN, tomorrow we will try something different and redo ligation on Monday
Mini-prep of 7 O/N colonies of K102003.1-K102003.7
Set up for sequencing
