Team:Heidelberg/Notebook/Visualization/Notebook/1stweek

From 2008.igem.org

(Difference between revisions)
(→Monday 01/09/2008)
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<!--[if lte IE 6]></td></tr></table></a><![endif]-->
<!--[if lte IE 6]></td></tr></table></a><![endif]-->
</li>
</li>
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<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/Visualization"><span><span>Visualization ></span></span><!--[if gt IE 6]><!--></a><!--<![endif]-->
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<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/visualization"><span><span>Visualization</span></span></a>
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<!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
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<ul class="SideMenu" id="MB1-DDM2-SM3">
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-
<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Protocols"><span>Final Protocols</span></a></li>
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-
<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Notebook"><span>Notebook</span></a></li>
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</ul>
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<!--[if lte IE 6]></td></tr></table></a><![endif]-->
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</li>
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<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/material"><span><span>Material & Methods</span></span></a>
<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/material"><span><span>Material & Methods</span></span></a>
<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/team_meetings"><span><span>Team Meetings</span></span></a>
<li><a href="https://2008.igem.org/Team:Heidelberg/Notebook/team_meetings"><span><span>Team Meetings</span></span></a>

Revision as of 15:54, 27 October 2008

Overview

Contents

First week

Monday 01/09/2008

  • Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.

First microscope test using MG1655 in 6 small chambers from Labtek:

3 types each type twice:
I - 0.1% agar in LB
II - 0.25% agar in LB
III - 0.5% agar in LB

View:

I II III
I II III

  • In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
  • Because the III. solution was more viscous 150 µl were necessary to cover the ground.
  • The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) They have been set under the media or in between.
  • About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
  • The chambers were incubated overnight at 37 °C. For microscope on the next day.

Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center.
-> In the further future 150 - 200 µl will be used.

Tuesday 02/09/2008

Wednesday 03/09/2008

Thursday 04/09/2008

Friday 05/09/2008

Saturday 06/09/2008

Sunday 07/09/2008

2ndWeek

Retrieved from "http://2008.igem.org/Team:Heidelberg/Notebook/Visualization/Notebook/1stweek"