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Team:ETH Zurich/Modeling/Switch Circuit
From 2008.igem.org
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=== diffusion of tet === | === diffusion of tet === | ||
| - | [[Image:diffusion_tet.JPG|left]]<br> | + | [[Image:diffusion_tet.JPG|left]]<br clear="all" /> |
=== binding of tetR to LacI-promotor and LacIIs-promotor === | === binding of tetR to LacI-promotor and LacIIs-promotor === | ||
| - | [[Image:binding_tetR_to_LacI.JPG|left]] | + | [[Image:binding_tetR_to_LacI.JPG|left]]<br clear="all" > |
=== binding of LacI and LacIIs to GFP-promotor === | === binding of LacI and LacIIs to GFP-promotor === | ||
| - | [[Image:binding_LacIIs_to_Pgfp.JPG|left]] | + | [[Image:binding_LacIIs_to_Pgfp.JPG|left]]<br clear="all" > |
| - | [[Image:binding_LacI_to_Pgfp.JPG|left]] | + | [[Image:binding_LacI_to_Pgfp.JPG|left]]<br clear="all" > |
=== binding of tet to tetR === | === binding of tet to tetR === | ||
| - | [[Image:binding_tet_to_tetR.JPG|left]] | + | [[Image:binding_tet_to_tetR.JPG|left]]<br clear="all" > |
=== binding of IPTG to LacI === | === binding of IPTG to LacI === | ||
| - | [[Image:binding_IPTG_to_LacI.JPG|left]] | + | [[Image:binding_IPTG_to_LacI.JPG|left]]<br clear="all" > |
=== transcription and translation of LacI === | === transcription and translation of LacI === | ||
| - | [[Image:transcription_of_LacI.JPG|left]] | + | [[Image:transcription_of_LacI.JPG|left]]<br clear="all" > |
| - | [[Image:translation_of_LacI.JPG|left]] | + | [[Image:translation_of_LacI.JPG|left]]<br clear="all" > |
=== transcription and translation of LacIIs === | === transcription and translation of LacIIs === | ||
| - | [[Image:transcription_of_LacIIs.JPG|left]] | + | [[Image:transcription_of_LacIIs.JPG|left]]<br clear="all" > |
| - | [[Image:translation_of_LacIIs.JPG|left]] | + | [[Image:translation_of_LacIIs.JPG|left]]<br clear="all" > |
=== transcription and translation of tetR === | === transcription and translation of tetR === | ||
| - | [[Image:transcription_of_tetR.JPG|left]] | + | [[Image:transcription_of_tetR.JPG|left]]<br clear="all" > |
| - | [[Image:translation_of_tetR.JPG|left]] | + | [[Image:translation_of_tetR.JPG|left]]<br clear="all" > |
=== transcription and translation of GFP === | === transcription and translation of GFP === | ||
| - | [[Image:transcription_of_gfp.JPG|left]] | + | [[Image:transcription_of_gfp.JPG|left]]<br clear="all" > |
| - | [[Image:translation_of_gfp.JPG|left]] | + | [[Image:translation_of_gfp.JPG|left]]<br clear="all" > |
=== dimerization of tetR === | === dimerization of tetR === | ||
| - | [[Image:dimerization_of_tetR.JPG|left]] | + | [[Image:dimerization_of_tetR.JPG|left]]<br clear="all" > |
=== dimerization and tetramerization of LacI and LacIIs === | === dimerization and tetramerization of LacI and LacIIs === | ||
| - | [[Image:dimerization_of_LacI.JPG|left]] | + | [[Image:dimerization_of_LacI.JPG|left]]<br clear="all" > |
| - | [[Image:dimerization_of_LacIIs.JPG|left]] | + | [[Image:dimerization_of_LacIIs.JPG|left]]<br clear="all" > |
| - | [[Image:tetramerization_of_LacI.JPG|left]] | + | [[Image:tetramerization_of_LacI.JPG|left]]<br clear="all" > |
| - | [[Image:tetramerization_of_LacIIs.JPG|left]] | + | [[Image:tetramerization_of_LacIIs.JPG|left]]<br clear="all" > |
== Implementation == | == Implementation == | ||
Revision as of 17:13, 27 October 2008
Switch CircuitDetailed Modeldiffusion of IPTG diffusion of tet binding of tetR to LacI-promotor and LacIIs-promotor binding of LacI and LacIIs to GFP-promotor  binding of tet to tetR binding of IPTG to LacI transcription and translation of LacI  transcription and translation of LacIIs  transcription and translation of tetR  transcription and translation of GFP  dimerization of tetR dimerization and tetramerization of LacI and LacIIs    ImplementationThe model of the switch circuit has been implemented using the Simbiology Toolbox in MATLAB. For the sake of simplicity, the effects of dimerization and dimerization/tetramerization of tetR and LacI/LacIIs have been neglected. So we did with the additional steps involving the RNApolymerase in the transcription of proteins and the ribosomes in the translation of proteins.  SimulationResults and DiscussionParametersIn this section you can find all the parameters used in the simulation.
References |
