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USTC/1 August 2008
From 2008.igem.org
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>[[Team:USTC|Home]] | [[Team:USTC/Notebook|Notebook]] | >[[Team:USTC|Home]] | [[Team:USTC/Notebook|Notebook]] | ||
| - | [[Team:USTC/Calendar-Home/31 July 2008|31 July 2008 <]]|[[Team:USTC/Calendar-Home/ | + | [[Team:USTC/Calendar-Home/31 July 2008|31 July 2008 <]]|[[Team:USTC/Calendar-Home/3 Aug 2008|> 3 Agu 2008]] |
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*digest the biobrick BBa_B0034/32/30 with SpeI and pstI. | *digest the biobrick BBa_B0034/32/30 with SpeI and pstI. | ||
*digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI. | *digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI. | ||
| + | *run gel and extract the digest DNA parts | ||
| + | *ligate B0034 + C0070-B0015,B0032+C0070,B0032+C0070. | ||
Revision as of 17:24, 27 October 2008
Experiments
- digest the biobrick BBa_B0034/32/30 with SpeI and pstI.
- digest the biobrick BBa_B0070 and C0070-B0015 with XbaI and pstI.
- run gel and extract the digest DNA parts
- ligate B0034 + C0070-B0015,B0032+C0070,B0032+C0070.
