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Team:Bay Area RSI/Project
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Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment. | Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment. | ||
| - | == | + | == ''''''Targeting Infarcted Cardiac Tissue''''''== |
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| - | === | + | === C-Reactive Protein Receptor Intein Mediated Signaling Circuit === |
| + | overview | ||
| + | signaling picture | ||
| + | construct image | ||
| + | === FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro === | ||
| + | description | ||
| + | picture | ||
| + | picture exp | ||
| + | === CRP activates GFP signaling upon FCGamma Binding === | ||
| + | picture | ||
| + | picture exp | ||
| + | === Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment === | ||
| - | + | Overview | |
| - | + | Construct image | |
| - | + | pics from slides | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| + | === Future Directions === | ||
== Results == | == Results == | ||
Revision as of 18:39, 27 October 2008
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Project Overview
Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment.
'Targeting Infarcted Cardiac Tissue'
C-Reactive Protein Receptor Intein Mediated Signaling Circuit
overview signaling picture construct image
FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro
description picture picture exp
CRP activates GFP signaling upon FCGamma Binding
picture picture exp
Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment
Overview Construct image pics from slides
