Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of AraC+pBAD+AID

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AraC+pBAD+AID to LB with ampicillin.

Preparation of OmpA-linker-alpha-linker

Michał K., Piotr

1. Digest of pLac_OmpA_alpha and OmpA-linker (BBa_K103006) with EcoRI and SacI (BamHI buffer).
2. Gel electrophoresis and gel-out of proper band 500 bp for OmpA-linker (BBa_K103006) lane and 3000 bp for pLac_OmpA_alpha lane.
3. Ligation of isolated DNA fragments.
4. Transformation of TOP10 with above ligation.
5. Tranformants planting on LB with kanamycin.