Team:Warsaw/Calendar-Main/8 October 2008

From 2008.igem.org

(Difference between revisions)
Line 45: Line 45:
<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
-
  primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+ AID (annealing temperature - 55&deg;C, 60 s of elongation step). </li>
+
  primers on colonies from plates with transformations <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A3>pSB1A3</a>+<a href=http://partsregistry.org/Part:BBa_K103001>AID(BBa_K103001)</a> (annealing temperature - 55&deg;C, 60 s of elongation step). </li>
<li>Inoculation of confirmed colonies to liquid LB + ampicillin. </li></ol>
<li>Inoculation of confirmed colonies to liquid LB + ampicillin. </li></ol>

Revision as of 20:22, 27 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Preparation of linker_alpha (BBa_K103009)

Piotr

  1. Isolation of plasmid from culture inoculated on previous day (pSB2K3 + linker_alpha (BBa_K103009)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of linker_omega (BBa_K103013)

Piotr

  1. Isolation of plasmid from culture inoculated on previous day (pSB2K3 + linker_omega (BBa_K103013)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmid from culture inoculated on previous day (pACYC177 + OmpA-linker-omega-linker (BBa_K103016)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found.

Preparation of vector for pT7 constructs

Michał K.

Inoculation of colonies from plate with ligation of pET15b+OmpA_omega without XbaI to liquid LB + ampicillin.

Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)

Piotr

Inoculation of colonies from plate with ligation of pSB2K3 + BBa_K103018 (without internal EcoRI site) to liquid LB + kanamycin.

Preparation of AID(BBa_K103001)

Michał K.

  1. Colony PCR with AIDlNrH and AIDpLinB primers on colonies from plates with transformations pSB1A3+AID(BBa_K103001) (annealing temperature - 55°C, 60 s of elongation step).
  2. Inoculation of confirmed colonies to liquid LB + ampicillin.

Preparation of AraC+pBAD+AID

Piotr

  1. Transformation of TOP10 with ligation pMPMT5+AID without EcoRI site.
  2. Tranformants planting on LB with tetracycline.