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Team:Edinburgh/Results
From 2008.igem.org
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== Tests and Results == | == Tests and Results == | ||
| - | === | + | ===Cellulolysis device=== |
| - | * | + | * '''''cenA''''' (endoglucanase) and '''''cex''''' (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site. |
| - | * | + | * '''''bglX''''' (beta glucosidase): Biobrick made. |
| - | * [[Team: | + | * '''''P<sub>cstA</sub>''''' (promoter): Biobrick made and tested - successful. |
| + | ** [[Team:Edinbrugh/Results/PcstA-xylE|''P<sub>cstA</sub>'' promoter assay with ''xylE'' as repoter]] | ||
| - | === | + | === Glycogenesis device === |
| - | * [[Team: | + | * '''''glgC''''' and '''''glgC16''''' (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful. |
| + | ** [[Team:Edinburgh/Results/Glycogen1|Glycogen Assay 1 (Qualitative)]] | ||
| + | ** [[Team:Edinburgh/Results/Glycogen2|Glycogen Assay 2 (Qualitative)]] | ||
| + | ** [[Team:Edinburgh/Results/Glycogen3|Glycogen Assay 3 (Quantitative)]] | ||
| + | |||
| + | === Starch synthesis device === | ||
| + | * '''''su1''''' and '''''iso2''''' (isoamylases): Work in progress - to be continued in an undergraduate honours project. | ||
| + | |||
| + | ===Lycopene generator === | ||
| + | * '''''dxs+crtE+crtB+crtI+crtY''''': Work in progress - encountered problem at final step. | ||
| + | |||
| + | ===Limonene synthase device=== | ||
| + | * '''''dxs+LIMS+appY''''': Biobrick made - still being tested (last test failed to detect limonene synthesis). | ||
Revision as of 20:43, 27 October 2008
Contents |
Tests and Results
Cellulolysis device
- cenA (endoglucanase) and cex (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
- bglX (beta glucosidase): Biobrick made.
- PcstA (promoter): Biobrick made and tested - successful.
Glycogenesis device
- glgC and glgC16 (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.
Starch synthesis device
- su1 and iso2 (isoamylases): Work in progress - to be continued in an undergraduate honours project.
Lycopene generator
- dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.
Limonene synthase device
- dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).
