Team:PennState/diauxie/progress

From 2008.igem.org

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   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/references">References</a></dd>
   <dd><a href="https://2008.igem.org/Team:PennState/diauxie/references">References</a></dd>
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  <h4>Hormone Biosensors</h4>
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  <h4><acronym title="Nuclear Hormone Receptor">NHR</acronym><br/>Biosensors</h4>
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   <dd><a href="hbintro" title="Intro to Endocrine Disruption, pseudoestrogens, pthalates, nuclear hormone receptors, and our goals">Introduction</a></dd>
   <dd><a href="hbintro" title="Intro to Endocrine Disruption, pseudoestrogens, pthalates, nuclear hormone receptors, and our goals">Introduction</a></dd>
   <dt>Smart Fold</dt>
   <dt>Smart Fold</dt>
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Overview</a></dd>
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Overview</a></dd>
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  <dd><a href="https://2008.igem.org/Team:PennState/smartfold/parts" title="Parts submitted to the registry for this project">Parts</a></dd>
 
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  <dd><a href="https://2008.igem.org/Team:PennState/smartfold/references">References</a></dd>
 
   <dt>Nuclear Fusion</dt>
   <dt>Nuclear Fusion</dt>
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/overview">Overview</a></dd>
   <dd><a href="https://2008.igem.org/Team:PennState/fusion/overview">Overview</a></dd>
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  <dd><a href="https://2008.igem.org/Team:PennState/fusion/parts" title="Parts submitted to the registry for this project">Parts</a></dd>
 
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  <dd><a href="https://2008.igem.org/Team:PennState/fusion/references">References</a></dd>
 
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Revision as of 20:56, 27 October 2008

Diauxie Elimination

Introduction
Implementation
Progress
Parts
References

NHR
Biosensors

Introduction
Smart Fold
Overview
Nuclear Fusion
Overview
Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Promoter pSB1A2 DH5α W3110 ΔxylB-G W3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.