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Team:Edinbrugh/Results/Glycogen 4
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Organisms: | Organisms: | ||
| - | * | + | * Sample 1: Control strain of ''E. coli'' containing a plasmid that should have no effect on glycogen levels. |
| - | * | + | * Sample 2: ''E. coli'' overexpressing ''glgC''. |
| - | * | + | * Sample 3: ''E. coli'' overexpressing ''glgC16''. |
Growth Conditions: | Growth Conditions: | ||
Latest revision as of 22:36, 27 October 2008
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Glycogen Assay 4 (Quantitative: Raman)
Raman spectroscopy was performed by Dr Rabah Mouras.
Experiment Design
See Glycogen Assay 3 for explanation.
Legend
Organisms:
- Sample 1: Control strain of E. coli containing a plasmid that should have no effect on glycogen levels.
- Sample 2: E. coli overexpressing glgC.
- Sample 3: E. coli overexpressing glgC16.
Growth Conditions:
- Condition A: normal growth medium (LB, or L-broth).
- Condition B: LB + 10 mM glucose.
- Condition C: LB + 25 mM glucose.
- Condition D: LB + 50 mM glucose.
It is expected that the presence of higher levels of glucose, up to a point, will increase the amount of glycogen produced by the cells.
Results
Analysis forthcoming.
