Team:Warsaw/Calendar-Main/14 October 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with overnight ligation - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a>_pLac_OmpA_ + alpha2.</li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with overnight ligation - <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> (with OmpA_linker and lactose promoter) + alpha2.</li>
<li> Tranformants plating on LB with kanamycin. </li>
<li> Tranformants plating on LB with kanamycin. </li>

Revision as of 23:43, 27 October 2008

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Preparation of linker_alpha (BBa_K103009)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3 + linker_alpha (BBa_K103009).
  2. Tranformants plating on LB with kanamycin.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day - pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - still no proper clones found.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Isolation of plasmid from culture inoculated on previous day - pSB1A3 carrying alpha_linker under PT7 (BBa_K103019).

Preparation of omega_linker under PT7 (BBa_K103020)

Michał K.

  1. Isolation of plasmid from culture inoculated on previous day (pSB2K3 + omega_linker under PT7 (BBa_K103020)).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Transformation of TOP10 with overnight ligation - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Tranformants plating on LB with ampicillin.

Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)

Piotr

  1. Transformation of TOP10 with overnight ligation - pSB2K3 (with OmpA_linker and lactose promoter) + alpha2.
  2. Tranformants plating on LB with kanamycin.

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