"
Team:Warsaw/Calendar-Main/15 October 2008
From 2008.igem.org
(Difference between revisions)
| Line 27: | Line 27: | ||
<h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></h3> | <h3>Preparation of <a href=http://partsregistry.org/Part:BBa_K103017>OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></h3> | ||
<h4>Michał K.</h4> | <h4>Michał K.</h4> | ||
| - | <p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> | + | <p> Inoculation of colonies from plate with ligation of <a href=http://partsregistry.org/Part:pSB2K3>pSB2K3</a> (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin. </p> |
</ol> | </ol> | ||
Revision as of 23:45, 27 October 2008
 |
|
||||||||
 |
|
||||||||
Preparation of linker_alpha (BBa_K103009)Michał K.Inoculation of colonies from plate with ligation of pSB2K3 + linker_alpha (BBa_K103009). Preparation of OmpA-linker-omega-linker (BBa_K103016)Piotr, Michał K.
Preparation of AID under pBAD/araC (BBa_K103002)Michał K.Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin. Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)Michał K.Inoculation of colonies from plate with ligation of pSB2K3 (with lactose promoter and OmpA_linker) + alpha2 to LB with kanamycin. |
|
