Team:University of Lethbridge/Notebook/Project3September

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m (link to go back to the main notebook)
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[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
[[Team:University_of_Lethbridge/Notebook|Back to The University of Lethbridge Main Notebook]]
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September 30, 2008
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'''Andrew'''
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PCR of genomic + rpsA DNA with two different sets of primers
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 +
Reaction Conditions:
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  - 1.0 uL Template DNA
 +
  - 5.0 uL 10x Buffer
 +
  - 2.0 uL 10 mM dNTPs
 +
  - 1.0 uL Forward Primer
 +
  - 1.0 uL Reverse Primer
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  - 0.5 uL Econo taq polymerase
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  - 39.5 uL Optima H2O
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 +
PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA
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 +
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Cycling Conditions:
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1. 94 C 2 min
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2. a. 94 C 15 sec
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    b. 47 C 15 sec
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    c. 72 C 15 sec
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3. Repeat step 2 for 25 cycles
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4.72 C 5 min

Revision as of 00:47, 28 October 2008

Back to The University of Lethbridge Main Notebook

September 30, 2008

Andrew

PCR of genomic + rpsA DNA with two different sets of primers

Reaction Conditions: 

 - 1.0 uL Template DNA
 - 5.0 uL 10x Buffer
 - 2.0 uL 10 mM dNTPs
 - 1.0 uL Forward Primer
 - 1.0 uL Reverse Primer
 - 0.5 uL Econo taq polymerase
 - 39.5 uL Optima H2O

PCR carried out for 1/10 and 1/100 dilutions of genomic DNA and rpsA DNA


Cycling Conditions: 

1. 94 C 2 min
2. a. 94 C 15 sec
   b. 47 C 15 sec
   c. 72 C 15 sec

3. Repeat step 2 for 25 cycles 4.72 C 5 min

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