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Team:Utah State/Project
From 2008.igem.org
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would allow our reporter to indicate when PHB production was maximized. | would allow our reporter to indicate when PHB production was maximized. | ||
| - | == | + | == Introduction == |
| + | === Polyhydroxybutyrate === | ||
| + | === PHB Metabolic Pathways === | ||
| + | === Problems with PHB === | ||
| + | === Green Fluorescent Protein === | ||
| - | == | + | == Methods == |
| + | === Organisms === | ||
| + | === Gel Electrophoresis === | ||
| + | === Polymerase Chain Reaction === | ||
| + | === GFP Correlation === | ||
| - | === | + | === BioBrick Production === |
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== Results == | == Results == | ||
Revision as of 02:22, 28 October 2008
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Overall project
The Utah State University iGEM team project is focused on creating an efficient system for production and monitoring PHA production in microorganisms. One goal of our research is to develop and optimize a method, using fluorescent proteins, for the detection of maximum product yield of polyhydroxybutyrate (PHB, a bioplastic) in recombinant E. coli and in Cupriavidus necator. In order to develop an optimal PHB detection system, we focused on the identification of the most efficient reporter genes, and the best promoter sequences that would allow our reporter to indicate when PHB production was maximized.
