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Team:Utah State/Modeling
From 2008.igem.org
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{| align="center" | {| align="center" | ||
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!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]] | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State|<font color="#ffffff">Home</font>]] | ||
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]] | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Team|<font color="#ffffff">The Team</font>]] | ||
| - | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="# | + | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Project|<font color="#99CCFF">The Project</font>]] |
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#ffffff">Parts</font>]] | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Parts|<font color="#ffffff">Parts</font>]] | ||
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Notebook|<font color="#ffffff">Notebook</font>]] | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Notebook|<font color="#ffffff">Notebook</font>]] | ||
| - | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Modeling|<font color="# | + | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Modeling|<font color="#ffffff">Protocols</font>]] |
!style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Links|<font color="#ffffff">Links</font>]] | !style="text-align:center; background-color:#212223; border-width:0px; padding:2px;"|[[Team:Utah_State/Links|<font color="#ffffff">Links</font>]] | ||
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| + | <!--- The Mission, Experiments ---> | ||
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body {background-image:url("https://static.igem.org/mediawiki/2008/6/63/Bkgnd14.gif");background-repeat:repeat; } | body {background-image:url("https://static.igem.org/mediawiki/2008/6/63/Bkgnd14.gif");background-repeat:repeat; } | ||
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| + | </style> | ||
</html> | </html> | ||
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| + | |||
| + | == '''Abstract''' == | ||
| + | |||
| + | The Utah State University iGEM team project is focused on | ||
| + | creating an efficient system for production and monitoring PHA | ||
| + | production in microorganisms. One goal of our research is to develop and | ||
| + | optimize a method, using fluorescent proteins, for the detection of | ||
| + | maximum product yield of polyhydroxybutyrate (PHB, a bioplastic) in | ||
| + | recombinant E. coli and in Cupriavidus necator. In order to develop an | ||
| + | optimal PHB detection system, we focused on the identification of the | ||
| + | most efficient reporter genes, and the best promoter sequences that | ||
| + | would allow our reporter to indicate when PHB production was maximized. | ||
| + | |||
| + | == Introduction == | ||
| + | |||
| + | === Polyhydroxybutyrate === | ||
| + | |||
| + | === PHB Metabolic Pathways === | ||
| + | |||
| + | === Problems with PHB === | ||
| + | |||
| + | === Green Fluorescent Protein === | ||
| + | |||
| + | == Methods == | ||
| + | |||
| + | === Organisms === | ||
| + | |||
| + | === Gel Electrophoresis === | ||
| + | |||
| + | === Polymerase Chain Reaction === | ||
| + | |||
| + | === GFP Correlation === | ||
| + | |||
| + | === BioBrick Production === | ||
| + | |||
| + | |||
| + | == Results == | ||
Revision as of 03:35, 28 October 2008
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| Home | The Team | The Project | Parts | Notebook | Protocols | Links |
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Contents |
Abstract
The Utah State University iGEM team project is focused on creating an efficient system for production and monitoring PHA production in microorganisms. One goal of our research is to develop and optimize a method, using fluorescent proteins, for the detection of maximum product yield of polyhydroxybutyrate (PHB, a bioplastic) in recombinant E. coli and in Cupriavidus necator. In order to develop an optimal PHB detection system, we focused on the identification of the most efficient reporter genes, and the best promoter sequences that would allow our reporter to indicate when PHB production was maximized.
