Team:PennState

Welcome to the Penn State iGEM 2008 team website. In this website we explain our project and the two strategies we took to construct a phthalate sensing bacteria.

If there are any questions or comments about the information on this site please contact me at gjt5001@psu.edu.

Smart Fold Reporter

Smart Fold Reporter is the subproject for the first strategy we are trying to express hPPARalpha in E. coli. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully epressed and used to transcriptionally report for the presence of phthalates.

Nuclear Fusion

Nuclear Fusion is our second approach to constructing a phthalate detection system in E. coli. In this project we use just the ligand binding domain of hPPARalpha fused to thymidylate synthase (TS). Binding of the phthalate ligand to this chimeric protein activates TS. When this construct is placed in a TS diffident strain, only E. coli in the presence of a hPPARalpha agonist will survive.

Quick Links

Table of our Contributions to the Registry

Interactive E. coLisa Schematic

The University of Calgary

Obrien Centre for the BHSC

Drew Endy On Synthetic Biology

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            <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Smart Fold Reporter</span>
              <hr />
-             <div style="float:left; margin:5px;"> <img src="http://parts.mit.edu/igem07/images/e/e3/GlowingBacteria.jpg" alt="laser shining on bacteria" /> </div>
+             <div style="float:left; margin:5px;"> <img src="picture here" alt="name it" /> </div>
-             <p> <em>Smart Fold Reporter</em> is the subproject for the first strategy we are trying to express hPPARalpha in <em>E. coli</em>. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully epressed and used to transcriptionally report for the presence of phthalates</p></td>
+             <p> <em>Smart Fold Reporter</em> is the subproject for the first strategy we are trying to express hPPARalpha in <em>E. coli</em>. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully epressed and used to transcriptionally report for the presence of phthalates.</p></td>
-           <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">evoGEM!</span>
+           <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Nuclear Fusion</span>
              <hr />
-             <div style="float:left; margin:5px;"> <img src="http://parts.mit.edu/igem07/images/0/0f/EvoGemSim4.jpg" alt="Screenshot from EvoGEM" /> </div>
+             <div style="float:left; margin:5px;"> <img src="picture here" alt="name it" /> </div>
-             <p>Evolutionary computer systems at their finest. Our EvoGEM project is a computer program that <em>evolves</em> combinations of iGEM BioBricks to attain a certain result. Curious? Checkout our evoGEM links to the left for more information.</p></td>
+             <p> <em> Nuclear Fusion</em> is our second approach to constructing a phthalate detection system in <em> E. coli</em>. In this project we use just the ligand binding domain of hPPARalpha fused to thymidylate synthase (TS). Binding of the phthalate ligand to this chimeric protein activates TS. When this construct is placed in a TS diffident strain, only <em>E. coli</em> in the presence of a hPPARalpha agonist will survive. </p></td>
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