Team:Michigan/8 October 2008
From 2008.igem.org
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==<B> Three Weeks to Send Parts.... </B>== | ==<B> Three Weeks to Send Parts.... </B>== | ||
- | *Tried transforming a few BioBricks that had GFP in them; didn't get many colonies at all... - grew up cultures of those colonies and re-transformed | + | *Tried [https://2008.igem.org/Team:Michigan/Notebook/TransformationProtocol <u>transforming</u>] a few BioBricks that had GFP in them; didn't get many colonies at all... - grew up cultures of those colonies and [https://2008.igem.org/Team:Michigan/Notebook/TransformationProtocol <u>re-transformed</u>] |
- | *pTRTp midiprep didn't work; weird band lengths came out gel; proceeding with a new strategy for our activator module: | + | *pTRTp midiprep didn't work; weird band lengths came out [https://2008.igem.org/Team:Michigan/Notebook/GelProtocol <u>gel</u>]; proceeding with a new strategy for our activator module: |
*We'll be using BBa R0010 (lac repressible promoter) and pCLOCK1 (nifHp in LeuLP) - we'll drop in NifA behind these promoters. We'll then transfer to a landing pad (in case of the lac promoter driving NifA) and then put this piece on the chromosome. | *We'll be using BBa R0010 (lac repressible promoter) and pCLOCK1 (nifHp in LeuLP) - we'll drop in NifA behind these promoters. We'll then transfer to a landing pad (in case of the lac promoter driving NifA) and then put this piece on the chromosome. | ||
*Work done: | *Work done: | ||
- | *Digested pCLOCK1 with NdeI/SpeI, NifA with NdeI/SpeI, another NifA sample with Xba/Spe, and BBaR0010 with Spe. Also digested BBa R0010 with Xba/Spe and our pCLOCK1 and pCLOCK2 midi-preps with EcorI/SpeI | + | *[https://2008.igem.org/Team:Michigan/Notebook/DigestProtocol <u>Digested</u>] pCLOCK1 with NdeI/SpeI, NifA with NdeI/SpeI, another NifA sample with Xba/Spe, and BBaR0010 with Spe. Also [https://2008.igem.org/Team:Michigan/Notebook/DigestProtocol <u>digested</u>] BBa R0010 with Xba/Spe and our pCLOCK1 and pCLOCK2 midi-preps with EcorI/SpeI |
- | *Transformed BioBricks in XL-1 blue competent cells | + | *[https://2008.igem.org/Team:Michigan/Notebook/TransformationProtocol <u>Transformed</u>] BioBricks in XL-1 blue competent cells |
*Picked/Grew up Cultures of colonies we got off our plates | *Picked/Grew up Cultures of colonies we got off our plates | ||
Latest revision as of 07:39, 28 October 2008
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October 8, 2008Three Weeks to Send Parts....
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Calendar
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