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Team:Heidelberg/Notebook/Visualization/Notebook/1stweek
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| + | [[Team:Heidelberg/Notebook/Visualization/Notebook|Overview]]<br> | ||
| + | |||
| + | =First week= | ||
| + | |||
| + | ==Monday 01/09/2008== | ||
| + | |||
| + | * Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %. | ||
| + | |||
| + | First microscope test using MG1655 in 6 small chambers from Labtek: | ||
| + | |||
| + | 3 types each type twice:<br> | ||
| + | I - 0.1% agar in LB<br> | ||
| + | II - 0.25% agar in LB<br> | ||
| + | III - 0.5% agar in LB<br> | ||
| + | |||
| + | View:<br> | ||
| + | |||
| + | I II III<br> | ||
| + | I II III<br> | ||
| + | |||
| + | * In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground. | ||
| + | |||
| + | * Because the III. solution was more viscous 150 µl were necessary to cover the ground. | ||
| + | |||
| + | * The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) They have been set under the media or in between. | ||
| + | |||
| + | * About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added. | ||
| + | |||
| + | * The chambers were incubated overnight at 37 °C. For microscope on the next day. | ||
| + | |||
| + | Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center.<br> | ||
| + | -> In the further future 150 - 200 µl will be used. | ||
| + | |||
| + | ==Tuesday 02/09/2008== | ||
| + | ==Wednesday 03/09/2008== | ||
| + | ==Thursday 04/09/2008== | ||
| + | ==Friday 05/09/2008== | ||
| + | ==Saturday 06/09/2008== | ||
| + | ==Sunday 07/09/2008== | ||
| + | |||
| + | [[Team:Heidelberg/Notebook/Visualization/Notebook/2ndweek|2ndWeek]] | ||
Revision as of 07:54, 28 October 2008
Contents |
First week
Monday 01/09/2008
- Preparation of TB media with an Agar concentration of 0.4 %, 0.2 % and 0.1 %.
First microscope test using MG1655 in 6 small chambers from Labtek:
3 types each type twice:
I - 0.1% agar in LB
II - 0.25% agar in LB
III - 0.5% agar in LB
View:
I II III
I II III
- In I and II each chamber was filled with just 100 µl LB agar to cover the whole ground.
- Because the III. solution was more viscous 150 µl were necessary to cover the ground.
- The bacteria were put into one corner of a chamber. (2 µl of a MG1655 overnight culture) They have been set under the media or in between.
- About that another 100 µl of a LB solution with 2 % agar were poured. After the second layer has solidified further 250 µl LB-media were added.
- The chambers were incubated overnight at 37 °C. For microscope on the next day.
Notes: Because of surface tensions the solutions tend to be thicker at the borders of the chamber than in the center.
-> In the further future 150 - 200 µl will be used.
