Team:Chiba/parts/BBa K084008

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===parts usage===
===parts usage===
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[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])
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[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)])
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[[Image:Tet-rhli chiba.gif]]
[[Image:Tet-lasi chiba.gif]]
[[Image:Tet-lasi chiba.gif]]
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日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
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日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
English:

Revision as of 11:32, 28 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements


parts usage

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]) Tet-rhli chiba.gif

Tet-lasi chiba.gif

日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。

English:

Experient

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.
  1. E.Coli strain JW1908 or XL10Gold.
  2. Reaction Temparature 37 or 30.
  3. Cell number.

Results

Fig.
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