Team:Chiba/AHL Receiver Phase

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Revision as of 11:33, 28 October 2008

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Signal Molecule Receiver Phase

Quorum_Sensing_Crosstalk

Design

Table.1 LuxR family gene

センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）

Experiment

Fig.R family crosstalk test

Sender
- AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 BBa_S03623 ]

Receivers
- LuxR

- RhlR[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]
- LasR[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]
- CinR[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]

Result

Plasmid_Copy_number

Design

Experiment

Result

LuxR_mutant

Design

Fig.6 Location of the LuxR mutant in LuxR

Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)

Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)

Experiment

Result

Home	The Team	The Project	Parts Submitted to the Registry	Reference	Notebook	Acknowledgements

@@ Line 24: / Line 24: @@
 *'''Sender'''
-**[http://partsregistry.org/Part:BBa_S03623  AHL autoinucer BBa_S03623 ]
+**AHL autoinucer[http://partsregistry.org/Part:BBa_S03623  BBa_S03623 ]
 [[Image:LuxI-sender Chiba.gif|left|]]
@@ Line 30: / Line 30: @@
 **LuxR
 [[Image:Non-AiiA-Receiver Chiba.gif]]
-**[http://partsregistry.org/Part:BBa_K084004 RhlR BBa_K084004]
+**RhlR[http://partsregistry.org/Part:BBa_K084004 BBa_K084004]
-**[http://partsregistry.org/Part:BBa_K084005 LasR BBa_K084005]
+**LasR[http://partsregistry.org/Part:BBa_K084005 BBa_K084005]
-**[http://partsregistry.org/Part:BBa_K084006 CinR BBa_K084006]
+**CinR[http://partsregistry.org/Part:BBa_K084006 BBa_K084006]