"
Team:Chiba/parts/BBa K084008
From 2008.igem.org
(Difference between revisions)
(→crosstalk experients) |
(→Method) |
||
| Line 29: | Line 29: | ||
#Inoculated them independently in liquid media. Incubated at 37°C 12h | #Inoculated them independently in liquid media. Incubated at 37°C 12h | ||
#Mixed them. | #Mixed them. | ||
| - | #Incubated at 30°C. | + | #Incubated at 30°C and 37°C. |
#Measured intensity of green fluorescence at regular time intervals. | #Measured intensity of green fluorescence at regular time intervals. | ||
Latest revision as of 11:52, 28 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
|---|
parts usage
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)])
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
Experient
Purpose
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
Method
- Transformed BBa_K084008 into E.coli strains(XL10GOLD or JW1908) and BBa_T9002 into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C and 37°C.
- Measured intensity of green fluorescence at regular time intervals.
more details
Results
Fig.
