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Team:Chiba/AHL Receiver Phase
From 2008.igem.org
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== Signal Molecule Receiver Phase== | == Signal Molecule Receiver Phase== | ||
| - | == | + | ==Quorum Sensing Crosstalk== |
===Design=== | ===Design=== | ||
[[Image:Table R chiba.gif|frame|left|Table.1 LuxR family gene]] | [[Image:Table R chiba.gif|frame|left|Table.1 LuxR family gene]] | ||
Revision as of 13:08, 28 October 2008
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Signal Molecule Receiver Phase
Quorum Sensing Crosstalk
Design
Table.1 LuxR family gene
センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）
Experiment
Fig.R family crosstalk test
- Sender
- AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]
- Receivers
- LuxR
- RhlR[http://partsregistry.org/Part:BBa_K084004 (BBa_K084004)]
- LasR[http://partsregistry.org/Part:BBa_K084005 (BBa_K084005)]
- CinR[http://partsregistry.org/Part:BBa_K084006 (BBa_K084006)]
- Reporter
- Plux-GFP[http://partsregistry.org/Part:BBa_J37032 (BBa_J37032)]
Result
Plasmid_Copy_number
Design
Experiment
Result
LuxR_mutant
Design
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)
Experiment
Result
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