Team:NTU-Singapore/Notebook/23 June 2008

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(New page: =Monday 23 June= ==Morning (9am-1230pm) *DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [[https://2008.igem.org/wiki/index.php?title=Team:NTU-Singapore/Note...)
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Monday 23 June

==Morning (9am-1230pm)

  • DNA extraction (using Miniprep kit) for 21 samples that Choon Kit inoculated on [[1]]
  • Digestion of the above 21 plasmids by EcoRI and PstI, followed by Gel electrophoresis check after 2 hours incubation.
  • Digestion LacI promoter insert using SpeI and EcoRI sequentially with QiAgen PCR purification kit in between.
  • Digestion of RBS 0034 vector using XbaI and EcoRI sequentially and QIAgen PCR purification kit.
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