Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K.

Inoculation of colonies from plate with ligation of pSB2K3 + alpha_linker under PT7 (BBa_K103019) to LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

Inoculation of colonies from plate with ligation of pSB1A3 + AID under pBAD/araC (BBa_K103002) to LB with ampicillin.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K., Piotr

1. Digest of pSB2K3 + OmpA_linker_alpha_linker under Plac (BBa_K103017) and OmpA-linker (BBa_K103006) with EcoRI and SacI (BamHI buffer).
2. Gel electrophoresis and gel-out of proper band 500 bp for OmpA-linker (BBa_K103006) lane and 3000 bp for PSB2K3 (+ alpha linker) lane Fig. 1.
3. Ligation of isolated DNA fragments.
4. Transformation of TOP10 with above ligation.
5. Tranformants plating on LB with kanamycin.
Fig. 1.EcoRI/SacI digests of plasmids pSB2K3 + OmpA_linker_alpha_linker under Plac (BBa_K103017) and OmpA_linker (BBa_K103006):
1. Marker
2.EcoRI/SacI digest of pSB2K3+OmpA_linker (BBa_K103006)
3.EcoRI/SacI digest of pSB2K3+OmpA_linker_alpha_linker under Plac (BBa_K103017)