Team:Chiba/AHL Receiver Phase

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Revision as of 16:05, 28 October 2008

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Signal Molecule Receiver Phase

Quorum Sensing Crosstalk

Design

Table.1 LuxR family gene

センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）

Experiment

Fig.R family crosstalk test

Sender
- AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]

Receivers
- LuxR

- RhlR[http://partsregistry.org/Part:BBa_K084004 (BBa_K084004)]

- LasR[http://partsregistry.org/Part:BBa_K084005 (BBa_K084005)]

- CinR[http://partsregistry.org/Part:BBa_K084006 (BBa_K084006)]

Reporter
- Plux-GFP[http://partsregistry.org/Part:BBa_J37032 (BBa_J37032)]

Result

Fig.　 Time Delay Test

Plasmid Copy number

Design

Experiment

Sender

AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]

Receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

Result

Fig.　 Time Delay Test

LuxR_mutant

Design

Fig.6 Location of the LuxR mutant in LuxR

Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)

Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))

私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)

Experiment

Result

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@@ Line 25: / Line 25: @@
 *'''Sender'''
 **AHL autoinucer[http://partsregistry.org/Part:BBa_S03623 (BBa_S03623) ]
-[[Image:LuxI-sender Chiba.gif|left|]]
+[[Image:LuxI-sender Chiba.gif|left|]]<br clear=all>
 *'''Receivers'''