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Newcastle University Wetlab/20 August 2008
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(New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Wednesday 20th August== * The dilute 2.2kb fragment was concentrated by repeated rounds of heating and cooling. This was purified to...) |
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==Wednesday 20th August== | ==Wednesday 20th August== | ||
| - | * The dilute 2.2kb fragment was concentrated by repeated rounds of heating and cooling. This was purified to see if any plasmid had been obtained. As the sample was in 500μl buffer, it was split into 5 x 100μl aliquots. Each was purified separatly and then the samples pooled afterwards to maximise the yield of DNA. This was then run on a [[gel]]. | + | * The dilute 2.2kb fragment was concentrated by repeated rounds of heating and cooling. This was purified to see if any plasmid had been obtained. As the sample was in 500μl buffer, it was split into 5 x 100μl aliquots. Each was purified separatly and then the samples pooled afterwards to maximise the yield of DNA. This was then run on a [[Agarose Gel Electrophoresis|gel]]. |
| - | * The gel showed no pGFPrrnB (restricted with EcoRI and NheI) and only a small amount of pGFPrrnB (restricted with EcoRI). Therefore pGFPrrnB and pUC57-ncl08 were [[restricted]] using EcoRI and NheI (as this also showed as absent on the gel). | + | * The gel showed no pGFPrrnB (restricted with EcoRI and NheI) and only a small amount of pGFPrrnB (restricted with EcoRI). Therefore pGFPrrnB and pUC57-ncl08 were [[Restricting Plasmids (Double Restriction)|restricted]] using EcoRI and NheI (as this also showed as absent on the gel). |
| - | * Following these restrictions, the samples were run on [[gel]] which again showed them to be absent. | + | * Following these restrictions, the samples were run on [[Agarose Gel Electrophoresis|gel]] which again showed them to be absent. |
Latest revision as of 16:25, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Wednesday 20th August
- The dilute 2.2kb fragment was concentrated by repeated rounds of heating and cooling. This was purified to see if any plasmid had been obtained. As the sample was in 500μl buffer, it was split into 5 x 100μl aliquots. Each was purified separatly and then the samples pooled afterwards to maximise the yield of DNA. This was then run on a gel.
- The gel showed no pGFPrrnB (restricted with EcoRI and NheI) and only a small amount of pGFPrrnB (restricted with EcoRI). Therefore pGFPrrnB and pUC57-ncl08 were restricted using EcoRI and NheI (as this also showed as absent on the gel).
- Following these restrictions, the samples were run on gel which again showed them to be absent.
