Team:PennState/diauxie/progress

From 2008.igem.org

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  <h4><acronym title="Nuclear Hormone Receptor">NHR Biosensors</acronym><br/></h4>
  <h4><acronym title="Nuclear Hormone Receptor">NHR Biosensors</acronym><br/></h4>
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   <dd><a href="hbintro" title="Intro to Endocrine Disruption, pseudoestrogens, pthalates, nuclear hormone receptors, and our goals">Introduction</a></dd>
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   <dd><a href="https://2008.igem.org/Team:PennState/NHR/introduction">NHR Introduction</a></dd>
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Phthalate Biosensor</a></dd>
   <dd><a href="https://2008.igem.org/Team:PennState/smartfold/overview">Phthalate Biosensor</a></dd>

Revision as of 16:27, 28 October 2008

Home The Team The Project Parts Notebook

Diauxie Elimination

Introduction
The System
Strategies
Progress
Conclusions
Parts
References

NHR Biosensors

NHR Introduction
Phthalate Biosensor
BPA Biosensor
 Progress & Results

We have completed our first set of cloning phases and are conducting our first round of testing and modification. Each test construct (promoter + GFP) has been cloned into pSB1A2 and transformed into DH5α, W3110 ∆xylB-G, and W3110 ∆xylB-R. Preliminary induction studies were run to find the optimal induction time and to see in what range OD and fluorescence were linearly related.

Promoter pSB1A2 DH5α W3110 ΔxylB-G W3110 ΔxylB-R
PN
P1
P3
P5

Test were then run to compare the level of induction between mixes of xylose, glucose and xylose, and glucose. The goal of this project is to obtain a noticeably higher level of induction when induced with xylose and glucose compared to the wildtype construct.

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