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Team:Hawaii/Protocols/Transformation E coli
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(New page: == Protocol == # Thawed cells on ice. # Transformed 50 μl cells w/ 1 μl plasmid # Incubated on ice 30 min. # Heat shocked 60 sec. in 42C water bath # Added 250 μl SOC # Incubated ...)
Newer edit →
(New page: == Protocol == # Thawed cells on ice. # Transformed 50 μl cells w/ 1 μl plasmid # Incubated on ice 30 min. # Heat shocked 60 sec. in 42C water bath # Added 250 μl SOC # Incubated ...)
Newer edit →
Revision as of 04:27, 24 June 2008
Protocol
- Thawed cells on ice.
- Transformed 50 μl cells w/ 1 μl plasmid
- Incubated on ice 30 min.
- Heat shocked 60 sec. in 42C water bath
- Added 250 μl SOC
- Incubated @ 37C for 1 hour with shaking (150 rpm)
- Plated 20 μl onto LB+spectinomycin plates
- Plate more if needed.
