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Sequence
From 2008.igem.org
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(New page: ==Sequencing Protocol== '''Protocol:''' Go to Genewiz.com to order sequencing. Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used. Depending on plasmid size...)
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(New page: ==Sequencing Protocol== '''Protocol:''' Go to Genewiz.com to order sequencing. Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used. Depending on plasmid size...)
Newer edit →
Revision as of 17:51, 28 October 2008
Sequencing Protocol
Protocol:
Go to Genewiz.com to order sequencing.
Sequencing primers should be diluted to 5uM; 4.0ul of 5uM primer should be used.
Depending on plasmid size and concentration (see table in Protocol.pdf), use up to 8ul of DNA; if less than 8ul, add autoclaved deionized water.
