Team:Warsaw/Calendar-Main/18 September 2008
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- | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/c/c3/Grad2_17_09.jpg" width= | + | <a name="fig1"><img src="https://static.igem.org/mediawiki/2008/c/c3/Grad2_17_09.jpg" width=180/></a><var><b>Fig. 1. </b>Gradient PCR (temperatures:55-75 °C)<br> |
1. Marker<br> | 1. Marker<br> | ||
2. 55 °C linker_alpha<br> | 2. 55 °C linker_alpha<br> | ||
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<p class="hide"><br> | <p class="hide"><br> | ||
- | <img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width= | + | <img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width=140/><var><b>Fig. 2.</b> PCR to obtain inserts<br> |
1. Marker<br> | 1. Marker<br> | ||
2. DeltaA<br> | 2. DeltaA<br> | ||
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4. OmpA_omega<br></var> | 4. OmpA_omega<br></var> | ||
</p> | </p> | ||
- | <a name="fig3"><img src="https://static.igem.org/mediawiki/2008/9/96/Go2_19_09.jpg" width= | + | <a name="fig3"><img src="https://static.igem.org/mediawiki/2008/9/96/Go2_19_09.jpg" width=180/></a><var><b>Fig. 3.</b> EcoRI/BcuI digest of pSB1A3 <br> |
1. Marker<br> | 1. Marker<br> | ||
2. Digested pSB1A3<br></var> | 2. Digested pSB1A3<br></var> | ||
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- | <a name="fig4"><img src="https://static.igem.org/mediawiki/2008/8/85/Go_1909_2008.jpg" width= | + | <a name="fig4"><img src="https://static.igem.org/mediawiki/2008/8/85/Go_1909_2008.jpg" width=180/></a><var><b>Fig. 4.</b> PCR to obtain linker_alpha for reisolation from agarose gel<br> |
1. Marker<br> | 1. Marker<br> | ||
2. linker_alpha<br></var> | 2. linker_alpha<br></var> | ||
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<p class="hide"><br> | <p class="hide"><br> | ||
- | <img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width= | + | <img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width=180/><var><b>Fig. 2.</b> PCR to obtain inserts<br> |
1. Marker<br> | 1. Marker<br> | ||
2. DeltaA<br> | 2. DeltaA<br> | ||
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- | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width= | + | <a name="fig2"><img src="https://static.igem.org/mediawiki/2008/d/d0/Go_17_09.jpg" width=220/></a><var><b>Fig. 2.</b> PCR products for reisolation from agarose gel<br> |
1. Marker<br> | 1. Marker<br> | ||
2. deltaA<br> | 2. deltaA<br> | ||
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and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (OmpA_linker - 500 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008#fig5">Fig. 5</a>.</li> | and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (OmpA_linker - 500 bp). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/18_September_2008#fig5">Fig. 5</a>.</li> | ||
- | <a name="fig5"><img src="https://static.igem.org/mediawiki/2008/6/6a/Go_29_09.jpg" width= | + | <a name="fig5"><img src="https://static.igem.org/mediawiki/2008/6/6a/Go_29_09.jpg" width=180/></a><var><b>Fig. 5. </b>SacI/NdeI digest of pET15b_OmpA_omega <br> |
1. Marker<br> | 1. Marker<br> | ||
2. digested pSB1A3<br></var> | 2. digested pSB1A3<br></var> |
Revision as of 18:13, 28 October 2008
'Hunter and prey' system tests: Competition testsWeronikaInoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_alpha + induction using 0.25mM iPTG MutD5 testingPiotrInoculation of mutD5 into 100 ml of LB and rendering them chemocompetent. Electroporation with pACYC177+OmpA_Z_alpha, pACYC177+OmpA_Z_omega, pACYC177+OmpA_A_omega and pACYC177+OmpA_omega_ΔA. Optimisation of primers for preparation of partsMichał K.
1. Marker 2. 55 °C linker_alpha 3. 60 °C linker_alpha 4. 65 °C linker_alpha 5. 70 °C linker_alpha Preparation of ΔA (BBa_K103003)Michał K.
1. Marker 2. Digested pSB1A3 Preparation of linker_alpha (BBa_K103009)Michał K.
1. Marker 2. linker_alpha Preparation of linker_omega (BBa_K103013)Michał K.
Preparation of OmpA-linker-omega-linker (BBa_K103016)Michał K.
Mentioned primers were used only for test purposes. During part preparation we've used another primers. Fig. 2. PCR products for reisolation from agarose gel 1. Marker 2. deltaA 3. omega 4. ompA_omega Preparation of OmpA-linker (BBa_K103006)Michał K.
1. Marker 2. digested pSB1A3
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