From 2008.igem.org
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| - | ==Description==
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| - | *Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study [http://www.ncbi.nlm.nih.gov/pubmed/9920267?dopt=Abstract (Kelly CG et al.; Nature Biotechnol. 1999)] reports that p1025 is good for your teeth. p1025 reduces oral colonization of ''Streptococcus mutans'', a tooth-decaying bacterium.
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| - | ** Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
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| - | ** Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of ''S. mutants'' to hydroxyapatite (HA) beads.
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| - | ** Stage 3: Expression of p1025 in Lactobacillus.
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| - | ** Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of ''S. mutants'' binding.
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| - | ==Notes==
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| - | * Group meetings every Friday at 4pm in 68-474 (except 7/11 and 8/1 in 68-674)
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| - | * Journal club meetings as needed every Friday at 11am in 68-474
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| | ==Information== | | ==Information== |
Revision as of 16:04, 24 June 2008
Notebook
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<LNCalendar></LNCalendar>
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150px <sitesearch>title=Search this project</sitesearch>
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Customize your entry pages 
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Information
- [[../Yogurt/Brainstorming |Brainstorming]]
- [[../Yogurt/Notes |Daily Notebook]]
- [[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]
- [[../Yogurt/lbulgaricus |Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols]]
- [[../Yogurt/toothbindingassay | Tooth binding assay protocol]]
- [[../Yogurt/Vocabulary List |Vocabulary List]]
Useful Links
- [http://www.biology.utah.edu/jorgensen/wayned/ape/ |DNA Plasmid Editor]
- [http://slam.bs.jhmi.edu/gd/ |Peptide Sequence Manipulations]
- Primer Analyzer Tool (check temperature & GC content) [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | One] [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/Default.aspx | Two]
- PMID 9920267
A synthetic peptide adhesion epitope as a novel antimicrobial agent.
- PMID 9062560
Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
- PMID 12788739
(a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
- PMID 11772607
Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.
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