Team:UCSF/Synthetic Chromatin Properties
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+ | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
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+ | <meta http-equiv="Content-Type" content="text/html; charset=UTF-8" /> | ||
+ | <title>Untitled Document</title> | ||
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+ | <body> | ||
+ | <p><a href="#">Design of our System (previous)</a></p> | ||
+ | <p> </p> | ||
+ | <h1 align="center">Synthetic Chromatin Bit (Part II)</h1> | ||
+ | <p align="center"> </p> | ||
+ | <blockquote> | ||
+ | <blockquote> | ||
+ | <h2 align="justify">Why use Chromatin as a Tool for Synthetic Biology?</h2> | ||
+ | <p align="justify">text here</p> | ||
+ | <h2 align="justify">Analysis of our Data</h2> | ||
+ | <p align="justify">Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).</p> | ||
+ | <p align="center"><img src="../../../Desktop/Analysis of data.jpg" width="750" height="458" /></p> | ||
+ | <p align="justify"> </p> | ||
+ | <h2 align="justify">The Properties of Our Synthetic Chromatin Bit</h2> | ||
+ | <p align="justify">We studied how our yeast synthetic chromatin system behaves...</p> | ||
+ | <p align="justify"> </p> | ||
+ | <h3 align="justify">1. Targeting of Sir2 leads to complete silencing of reporter</h3> | ||
+ | <p align="justify">text here</p> | ||
+ | <p align="center"><img src="minus galactose system.png" width="550" height="159" /></p> | ||
+ | <p align="center"><img src="../../../Desktop/plus galactose system.png" width="550" height="217" /></p> | ||
+ | <p align="justify"> </p> | ||
+ | <blockquote> | ||
+ | <p align="justify"><strong>RESULT 1:</strong></p> | ||
+ | </blockquote> | ||
+ | <p align="justify"> </p> | ||
+ | <p align="center"><img src="Basic bit.jpg" width="500" height="359" /></p> | ||
+ | <p align="justify">Similar results were obtained for other promoters (e.g. Fig1 P, see below).</p> | ||
+ | <h3 align="justify">2. Dominant over Transcription Factors</h3> | ||
+ | <blockquote> | ||
+ | <p align="justify">text here</p> | ||
+ | <p align="justify"> </p> | ||
+ | <p align="center"><img src="Galactose plus pheromone.png" width="550" height="169" /></p> | ||
+ | <p align="center"> </p> | ||
+ | <p align="center"><img src="Plus galactose plus pheromone.png" width="550" height="212" /></p> | ||
+ | </blockquote> | ||
+ | <p align="justify"> </p> | ||
+ | <blockquote> | ||
+ | <p align="justify"><strong>RESULT 2</strong>:</p> | ||
+ | </blockquote> | ||
+ | <p align="justify"> </p> | ||
+ | <blockquote> | ||
+ | <p align="justify"><img src="Gal on Fig1028.png" width="669" height="402" /></p> | ||
+ | <p align="justify">text here</p> | ||
+ | </blockquote> | ||
+ | <h3 align="justify">3. Regional Silencing</h3> | ||
+ | <blockquote> | ||
+ | <p align="justify">text here</p> | ||
+ | <p align="center"><img src="Regional silencing1028.png" width="400" height="38" /></p> | ||
+ | </blockquote> | ||
+ | <p align="justify"> </p> | ||
+ | <blockquote> | ||
+ | <p align="justify"><strong>RESULT 3:</strong></p> | ||
+ | <p align="justify">text</p> | ||
+ | </blockquote> | ||
+ | <p align="center"><img src="Regional_silencing.png" width="750" height="319" /></p> | ||
+ | <p align="left"> </p> | ||
+ | <h3 align="justify">4. Binary</h3> | ||
+ | <blockquote> | ||
+ | <p align="justify">text here</p> | ||
+ | </blockquote> | ||
+ | <h3 align="justify"> </h3> | ||
+ | <h3 align="justify">5. Memory</h3> | ||
+ | <blockquote> | ||
+ | <p align="justify">text here</p> | ||
+ | <p align="justify"><strong>RESULT 5:</strong></p> | ||
+ | <p align="center"><img src="Time-course1028a.png" width="750" height="252" /></p> | ||
+ | <p align="justify"> </p> | ||
+ | <p align="center"><img src="Time-course1028b.png" width="525" height="253" /></p> | ||
+ | <p align="justify"> </p> | ||
+ | text here | ||
+ | <p align="right"> </p> | ||
+ | <p align="right"><a href="#">Higher-Order Systems (next)</a></p> | ||
+ | </blockquote> | ||
+ | </blockquote> | ||
+ | </blockquote> | ||
+ | </body> | ||
+ | </html> | ||
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> |
Revision as of 21:06, 28 October 2008
Design of our System (previous)
Synthetic Chromatin Bit (Part II)
Why use Chromatin as a Tool for Synthetic Biology?
text here
Analysis of our Data
Single-cell analysis was done using flow-cytometry. Flow cytometry measurements were taken using a BD LSR-II flow cytometer (BD Biosciences). For each sample, 10,000 cells were counted, and GFP fluorescence was measured by exciting at 488 nm with a 100 mW Coherent Sapphire laser. Cells that were positive for GFP are seen in the microscope images on the lower right panel and their predicted population distribution is shown in the green curve in the ficticious graph in the left (below).
The Properties of Our Synthetic Chromatin Bit
We studied how our yeast synthetic chromatin system behaves...
1. Targeting of Sir2 leads to complete silencing of reporter
text here
RESULT 1:
Similar results were obtained for other promoters (e.g. Fig1 P, see below).
2. Dominant over Transcription Factors
text here
RESULT 2:
text here
3. Regional Silencing
text here
RESULT 3:
text
4. Binary
text here
5. Memory
text here
RESULT 5:
text here
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