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Template:Team:UC Berkeley/Notebook/SC construction
From 2008.igem.org
(Difference between revisions)
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SC003 Reverse Biobricking of <AP-tag! | SC003 Reverse Biobricking of <AP-tag! | ||
ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg | ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg | ||
| + | |||
| + | </pre> | ||
| + | |||
| + | == pBca1256-K112602 == | ||
| + | <pre> | ||
| + | Construction of <FLAG-tag> basic part K112602 | ||
| + | |||
| + | Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) | ||
| + | Product is pBca1256-K112602 | ||
| + | ------------------------------------------------------------- | ||
| + | SC004 Forward Biobricking of <FLAG-tag> | ||
| + | cgataGAATTCatgAGATCTgactacaaggatgacgacg | ||
| + | SC005 Reverse Biobricking of <FLAG-tag> | ||
| + | ccagtGGATCCcttgtcgtcgtcatccttgtagtc | ||
| + | |||
| + | </pre> | ||
| + | |||
| + | == pBca1256-K112603 == | ||
| + | <pre> | ||
| + | |||
| + | Construction of <FLAG-tag! basic part K112603 | ||
| + | |||
| + | Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) | ||
| + | Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) | ||
| + | Product is pBca1256-K112603 | ||
| + | ------------------------------------------------------------- | ||
| + | SC004 Forward Biobricking of <FLAG-tag! | ||
| + | cgataGAATTCatgAGATCTgactacaaggatgacgacg | ||
| + | SC006 Reverse Biobricking of <FLAG-tag! | ||
| + | ccagtGGATCCttacttgtcgtcgtcatccttgtagtc | ||
| + | |||
| + | </pre> | ||
Revision as of 18:55, 24 June 2008
Contents |
pBca1256-K112600
Construction of <AP-tag> basic part K112600 Wobble PCR SC001/SC002 (66 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112600 ---------------------------------------------------- SC001F Forward Biobricking of <AP-tag> cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC002R Reverse Biobricking of <AP-tag> ccagtGGATCCttcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112601
Construction of <AP-tag! basic part K112601 Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112601 -------------------------------------------------- SC001 Forward Biobricking of <AP-tag! cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag SC003 Reverse Biobricking of <AP-tag! ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg
pBca1256-K112602
Construction of <FLAG-tag> basic part K112602 Wobble PCR SC004/SC005 (45 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112602 ------------------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag> cgataGAATTCatgAGATCTgactacaaggatgacgacg SC005 Reverse Biobricking of <FLAG-tag> ccagtGGATCCcttgtcgtcgtcatccttgtagtc
pBca1256-K112603
Construction of <FLAG-tag! basic part K112603 Wobble PCR SC004/SC006 (48 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2227+ 910) Product is pBca1256-K112603 ------------------------------------------------------------- SC004 Forward Biobricking of <FLAG-tag! cgataGAATTCatgAGATCTgactacaaggatgacgacg SC006 Reverse Biobricking of <FLAG-tag! ccagtGGATCCttacttgtcgtcgtcatccttgtagtc
