Team:PennState

Welcome to the Penn State iGEM 2008 team website. We have been working hard at a few different projects for this year's competition. Starting this summer we began working trying to create different types of biosensors that use human nuclear hormone receptors to recognize potentially harmful ligands. We also have been finishing up one of last year's projects which is amied at more reaching efficent bioproduction by altering how E. Coli selects between utilizing 5 and 6 carbon sugars. Please explore our website to find out more about us and our projects!

If there are any questions or comments about the information on this site please contact us at gjt5001@psu.edu.

Hormone Prescreening E. coli

Our project aims to construct a biosensor which will ultimately serve as a prescreening tool to detect the presence of phthalates in water sample. Recently phthalates have been shown to cause negative health effect in humans; some phthalates are even though to be carcinogenic. Analytical detection methods for these compounds are compound specific as well as very costly. Having a simple, and cheap tool to screen for phthalates as a general class of compounds would eliminate some of the cost and time involved in current detection methods.

We use the natural human nuclear hormone receptor protein that recognizes phthalates as an agonist (hPPARα), and expressing it heterologously in Escherichia Coli. Because of the complexity of this mammalian protein, expressing it in a prokaryote is difficult. We have two different strategies to express hPPARα and using it to detect phthalates in E. Coli.

Smart Fold Reporter

Smart Fold Reporter is the subproject for the first strategy we are trying to express hPPARα in E. coli. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully expressed and used to transcriptionally report for the presence of phthalates.

Nuclear Fusion

Nuclear Fusion is our second approach to constructing a phthalate detection system in E. coli. In this project we use just the ligand binding domain of hPPARα fused to thymidylate synthase (TS). Binding of the phthalate ligand to this chimeric protein activates TS. When this construct is placed in a TS diffident strain, only E. coli in the presence of a hPPARalpha agonist will survive.

Diauxie Elimination: Two spoons full of sugar.

[img] Cellulosic biomass is an aboundant and inexpensive energy source, coming from plant waste: ideal for Ethanol production through fermentation. However, biomass contains glucose and xylose sugars in relatively equal ratios, while e. coli preferentially metabolizes glucose before any other sugar. In this project we attempt to eliminate this phenomenon, called diauxie, and get our cells to utilize both sugars at the same time. Solving this problem will lead to more efficent use of cellulosic biomass including moving towards the future of bioproduction: continous processes.

Quick Links

Table of our Contributions to the Registry

Interactive E. coLisa Schematic

The University of Calgary

Obrien Centre for the BHSC

Drew Endy On Synthetic Biology

Sponsors for our team! Thanks so much!

@@ Line 223: / Line 223: @@
 <td valign="top"><td style="padding-top:30px; padding-right:30px" valign="top" width="90%"><span style="font-size:14pt">Hormone Prescreening <em>E. coli</em></span>
        <hr />
-       <p>Our project aims to construct a bio sensor which will ultimately serve as a pre screening tool to detect the presence of phthalates in water sample. Recently phthalates have been shown to cause negative health effect in humans; some phthalates are even though to be carcinogenic. Analytical detection methods for these compounds are compound specific as well as very costly. Having a simple, and cheap tool to screen for phthalates as a general class of compounds would eliminate some of the cost and time involved in current detection methods.</p>
+       <p>Our project aims to construct a biosensor which will ultimately serve as a prescreening tool to detect the presence of phthalates in water sample. Recently phthalates have been shown to cause negative health effect in humans; some phthalates are even though to be carcinogenic. Analytical detection methods for these compounds are compound specific as well as very costly. Having a simple, and cheap tool to screen for phthalates as a general class of compounds would eliminate some of the cost and time involved in current detection methods.</p>
        <p> We use the natural human nuclear hormone receptor protein that recognizes phthalates as an agonist (hPPARα), and expressing it heterologously in <em>Escherichia Coli</em>. Because of the complexity of this mammalian protein, expressing it in a prokaryote is difficult. We have two different strategies to express hPPARα and using it to detect phthalates in <em>E. Coli</em>.  </p>
        <table>
@@ Line 230: / Line 230: @@
              <hr />
              <img src="picture here" alt="[img]" style="float:left; margin:5px;"/>
-             <p> <em>Smart Fold Reporter</em> is the subproject for the first strategy we are trying to express hPPARalpha in <em>E. coli</em>. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully epressed and used to transcriptionally report for the presence of phthalates.</p></td>
+             <p> <em>Smart Fold Reporter</em> is the subproject for the first strategy we are trying to express hPPARα in <em>E. coli</em>. This project uses altered growth conditions such that this nuclear hormone receptor protein can be successfully expressed and used to transcriptionally report for the presence of phthalates.</p></td>
            <td style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size:18px">Nuclear Fusion</span>
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      <td colspan="2" style="padding-top:30px; padding-right:30px" valign="top" width="45%"><span style="font-size: 14pt">Diauxie Elimination: <em>Two</em> spoons full of sugar.</span>
        <hr />
-       <p><img src="picture here" alt="[img]" style="float:left; margin:5px;"/>Cellulosic biomass is an aboundant and inexpensive energy source, coming from plant waste and the like: ideal for Ethanol production through fermentation. However, biomass contains glucose and xylose sugars in relatively equal ratios, while <em>e. coli</em> preferentially metabolizes glucose before any other sugar. In this project we attempt to eliminate this phenomenon, called <em>diauxie</em>, and get our cells to utilize both sugars at the same time. Solving this problem will lead to more efficent use of cellulosic biomass including moving towards the future of bioproduction: continous processes.</p>
+       <p><img src="picture here" alt="[img]" style="float:left; margin:5px;"/>Cellulosic biomass is an aboundant and inexpensive energy source, coming from plant waste: ideal for Ethanol production through fermentation. However, biomass contains glucose and xylose sugars in relatively equal ratios, while <em>e. coli</em> preferentially metabolizes glucose before any other sugar. In this project we attempt to eliminate this phenomenon, called <em>diauxie</em>, and get our cells to utilize both sugars at the same time. Solving this problem will lead to more efficent use of cellulosic biomass including moving towards the future of bioproduction: continous processes.</p>
 </td>
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