Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
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- | + | <table class="month"><tr> | |
+ | <tdalign=center><h2>Table of contents</h2></td><td> </td></tr> | ||
+ | <td width=70% valign=top style="border-left-style: grooved"> | ||
+ | <h3><a onClick=switchMenu('one-armed-vect')> Preparation of one-armed bandit vectors</a></h3> | ||
+ | |||
+ | <div name="menu_item" style="display: none" id="one-armed-vect" > | ||
+ | <ol> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=6_May_2008&arg1=7_May_2008&arg2=9_May_2008&arg3=12_May_2008&arg4=13_May_2008&arg5=14_May_2008&name=Preparation%20of%20pMPMT5%20AID%20construct>pMPMT5+AID</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=3_June_2008&arg1=4_June_2008&arg2=5_June_2008&name=Preparation%20of%20pMPMT5%20T7%20construct>pMPMT5-T7</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=16&arg0=5_May_2008&arg1=6_May_2008&arg2=8_May_2008&arg3=13_May_2008&arg4=14_May_2008&arg5=15_May_2008&arg6=16_May_2008&arg7=19_May_2008&arg8=20_May_2008&arg9=21_May_2008&arg10=22_May_2008&arg11=23_May_2008&arg12=24_May_2008&arg13=26_May_2008&arg14=27_May_2008&arg15=28_May_2008&name=Preparation%20of%20pMPMT5-AID%20T7%20and%20pMPMT5%20AID-T7>pMPMT5+AID+T7 and pMPM+AID-T7</a></li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=2_June_2008&arg1=3_June_2008&arg2=4_June_2008&arg3=5_June_2008&arg4=6_June_2008&arg5=9_June_2008&name=Preparation%20of%20pMPMT5-AID%20AIDT7%20construct>pMPMT5+AID+AID-T7</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=25_June_2008&arg1=26_June_2008&arg2=27_June_2008&arg3=30_June_2008&arg4=1_July_2008&arg5=2_July_2008&arg6=3_July_2008&arg7=4_July_2008&name=Change%20of%20the%20reporter%20from%20pZC320%20with%20B-galactosidase%20to%20GFP%20or%20RFP> Change of the reporter from pZC320 with B-galactosidase to GFP or RFP</a></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <h3><a OnClick=switchMenu('one-armed-tests')> One-armed bandit tests</a></h3> | ||
+ | |||
+ | <div name="menu_item" style="display: none" id="one-armed-tests"> | ||
+ | <ol> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=19_May_2008&arg1=20_May_2008&arg2=21_May_2008&name=Rifampicin%20test%20%231>Rifampicin Test #1 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=22_May_2008&arg1=23_May_2008&arg2=24_May_2008&name=Rifampicin%20test%20%232>Rifampicin Test #2 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=28_May_2008&arg1=29_May_2008&arg2=2_June_2008&name=Rifampicin%20test%20%233>Rifampicin Test #3 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=2_June_2008&arg1=5_June_2008&arg2=6_June_2008&arg3=11_June_2008&arg4=12_June_2008&arg5=13_June_2008&arg6=16_June_2008&name=Blue/white%20and%20rifampicin%20test%20%231>Blue/white and Rifampicin test#1 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=17_June_2008&arg1=18_June_2008&arg2=19_June_2008&arg3=23_June_2008&name=Blue/white%20and%20rifampicin%20test%20%232>Blue/white and Rifampicin test#2 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=29_August_2008&arg1=30_August_2008&arg2=31_August_2008&arg3=1_September_2008&name=Blue-white%20screening%20and%20rifampicin%20test%20in%20GM2163>Blue-white screening and rifampicin test in GM2163 </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=17_September_2008&arg1=18_September_2008&arg2=19_September_2008&arg3=22_September_2008&arg4=23_September_2008&arg5=24_September_2008&arg6=25_September_2008&arg7=29_September_2008&name=MutD5%20testing>MutD5 testing </a></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | <h3><a OnClick=switchMenu('hunter-vect')>Preparation of vectors for 'hunter' part of 'hunter and prey'</a></h3> | ||
+ | <div name="menu_item" id="hunter-vect" style="display: none"> | ||
+ | <ol> | ||
+ | <li class="nb_toc1"><a OnClick=switchMenu('prot_A','submenu_item')>Preparation of vectors with protein A derivatives fusions</a></li> | ||
+ | <div name="submenu_item" id="prot_A" style="display: none"> | ||
+ | <ul> | ||
+ | <li><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=10_June_2008&name=Design%20of%20primers%20for%20fusion%20of%20protein%20A%2C%20OmpA%20and%20alpha%20and%20omega%20fragments%20of%20beta-lactamase>Design of primers for fusion of protein A, OmpA and alpha and omega fragments of β-lactamase</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=20_June_2008&arg1=23_June_2008&arg2=24_June_2008&arg3=25_June_2008&arg4=26_June_2008&name=Preparation%20of%20alpha-A%20and%20omega-A%20fusions>Preparation of alpha-A and omega-A fusions</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=27_June_2008&arg1=30_June_2008&arg2=1_July_2008&arg3=3_July_2008&name=Cloning%20alpha-A%20and%20omega-A%20fusions%20on%20pKS>Cloning alpha-A and omega-A fusions on pKS </a></li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=7_July_2008&arg1=8_July_2008&arg2=14_July_2008&arg3=15_July_2008&arg4=16_July_2008&arg5=21_July_2008&arg6=22_July_2008&arg7=23_July_2008&arg8=28_July_2008&name=Cloning%20omega-A%20fusion%20on%20pKS%20%28second%20attempt%29>Cloning omega-A fusion on pKS (second attempt)</a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Preparation%20of%20construct%20pKS%20with%20A%20protein>Preparation of construct pKS with A protein </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=14_July_2008&arg1=15_July_2008&name=Preparation%20of%20alpha%20A%20conctruct>Preparation of alpha A conctruct </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=21_July_2008&arg1=22_July_2008&arg2=23_July_2008&arg3=24_July_2008&arg4=25_July_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20OmpA%20constructs>Cloning of protein A DNA to OmpA constructs </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_July_2008&arg1=30_July_2008&arg2=31_July_2008&name=Cloning%20of%20omega_%26Delta%3BA%20DNA%20fragment%20to%20pACYC177%20OmpA_alpha>Cloning of omega_ΔA DNA fragment to pACYC177 OmpA_alpha </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_July_2008&arg1=31_July_2008&arg2=1_August_2008&arg3=4_August_2008&arg4=5_August_2008&name=Cloning%20of%20truncated%20fragment%20of%20protein%20A%20%28%26Delta%3BA%29>Cloning of truncated fragment of protein A (ΔA) </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid>Cloning of protein A DNA to GeneArt plasmid </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct">Preparing pACYC177 OmpA_omega_ΔA construct</a> | ||
+ | </li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_August_2008&arg1=19_August_2008&arg2=20_August_2008&arg3=21_August_2008&arg4=22_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20GeneArt%20plasmid">Cloning of protein A DNA to GeneArt plasmid</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=22_September_2008&arg1=23_September_2008&arg2=24_September_2008&arg3=25_September_2008&arg4=26_September_2008&arg5=27_September_2008&arg6=30_September_2008&name=Mutagenesis%20of%20protein%20A">Mutagenesis of protein A</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=24_September_2008&arg1=25_September_2008&name=Preparation%20of%20alpha_A%20construct">Preparation of alpha_A construct</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&arg3=7_August_2008&name=Preparing%20pACYC177%20OmpA_omega_%26Delta%3BA%20construct>Preparing pACYC177 OmpA_omega_ΔA construct </a></li> | ||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | <li class="nb_toc1"><a OnClick=switchMenu('prot_Z','submenu_item')>Preparation of vectors with protein Z derivatives fusions</a></li> | ||
+ | <div name="submenu_item" id="prot_Z" style="display: none"> | ||
+ | <ul> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega>Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=13_August_2008&arg1=14_August_2008&arg2=18_August_2008&arg3=19_August_2008&arg4=20_August_2008&arg5=21_August_2008&arg6=22_August_2008&arg7=25_August_2008&arg8=26_August_2008&name=Cloning%20of%20alpha%20to%20pACYC177%20OmpA_A_omega%20and%20pACYC177%20OmpA_Z_omega">Cloning of alpha to pACYC177 OmpA_A_omega and pACYC177 OmpA_Z_omega</a></li> | ||
+ | |||
+ | </ul></div> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=11&arg0=13_June_2008&arg1=16_June_2008&arg2=17_June_2008&arg3=18_June_2008&arg4=19_June_2008&arg5=20_June_2008&arg6=23_June_2008&arg7=24_June_2008&arg8=25_June_2008&arg9=26_June_2008&arg10=27_June_2008&name=Preparation%20of%20constructs%3A%20OmpA_alpha%20and%20OmpA_omega>Preparation of constructs: OmpA_alpha and OmpA_omega</a></li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=7_July_2008&arg1=8_July_2008&arg2=9_July_2008&arg3=10_July_2008&arg4=11_July_2008&arg5=12_July_2008&name=Preparation%20of%20constructs%3A%20OmpA_alpha%20and%20OmpA_omega%20%232>Preparation of constructs: OmpA_alpha and OmpA_omega #2 </a></li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | <h3><a OnClick=switchMenu('prey-vect')>Preparation of vectors for 'prey' part of 'hunter and prey</a></h3> | ||
+ | <div name="menu_item" id="prey-vect" style="display: none"> | ||
+ | <ol> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=30_June_2008&arg1=1_July_2008&arg2=2_July_2008&arg3=3_July_2008&arg4=4_July_2008&name=Preparation%20of%20constructs%20on%20pET15b%3A%20OmpA_alpha%20and%20OmpA_omega>Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=17_July_2008&arg1=18_July_2008&arg2=21_July_2008&arg3=22_July_2008&arg4=23_July_2008&arg5=24_July_2008&arg6=25_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-omega%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid>Cloning of protein A DNA to pET15b OmpA-alpha plasmid </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=14_July_2008&arg1=15_July_2008&arg2=16_July_2008&arg3=17_July_2008&arg4=18_July_2008&arg5=19_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20OmpA%20constructs>Cloning of protein Z DNA to OmpA constructs </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=9_July_2008&arg1=10_July_2008&arg2=11_July_2008&arg3=12_July_2008&name=Cloning%20of%20protein%20Z%20DNA%20to%20pET15b-OmpA-alpha%20in%20place%20of%20OmpA>Cloning of protein Z DNA to pET15b-OmpA-alpha in place of OmpA </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=19_August_2008&arg1=22_August_2008&arg2=25_August_2008&arg3=26_August_2008&arg4=27_August_2008&arg5=28_August_2008&arg6=29_August_2008&name=Cloning%20of%20protein%20A%20DNA%20to%20pET15b%20OmpA-alpha%20plasmid">Cloning of protein A DNA to pET15b OmpA-alpha plasmid</a></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | <h3><a OnClick=switchMenu('prey-purif')>Purification of prey proteins</a></h3> | ||
+ | <div name="menu_item" id="prey-purif" style="display: none"> | ||
+ | <ol> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega>Purification of proteins: Z-alpha and Z-omega </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008& | ||
+ | amp;arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha">Purification of proteins: A-alpha</a></li> | ||
+ | |||
+ | |||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <h3><a OnClick="switchMenu('hunter-tests')">'Hunter and prey' tests</h3> | ||
+ | <div name="menu_item" id="hunter-tests" style="display: none"> | ||
+ | <ol> | ||
+ | <li class="nb_toc1"><a OnClick="switchMenu('expr-tests','submenu_item')">Protein expression tests</li> | ||
+ | <div name="submenu_item" id="expr-tests" style="display: none"> | ||
+ | <ul> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=4_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20and%20OmpA_A_alpha%20expression>Checking OmpA_omega_ΔA_alpha and OmpA_A_alpha expression </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=5_August_2008&name=Checking%20OmpA_omega_%26Delta%3BA_alpha%20expression>Checking OmpA_omega_ΔA_alpha expression </a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | <li class="nb_toc1"><a OnClick="switchMenu('resist-tests','submenu_item')">Ampicillin-resistance tests</li> | ||
+ | <div name="submenu_item" id="resist-tests" style="display: none"> | ||
+ | <ul> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium>Tests for ampicillin resistance given by protein added to medium </a></li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=4_August_2008&arg1=5_August_2008&arg2=6_August_2008&name=Checking%20if%20OmpA_omega_%26Delta%3BA_alpha%20gives%20ampicillin%20resistance>Checking if OmpA_omega_ΔA_alpha gives ampicillin resistance </a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=19_August_2008&arg1=20_August_2008&arg2=21_August_2008&arg3=22_August_2008&arg4=25_August_2008&arg5=26_August_2008&arg6=27_August_2008&arg7=9_September_2008&arg8=10_September_2008&arg9=11_September_2008&name=Tests%20for%20ampicillin%20resistance%20given%20by%20protein%20added%20to%20medium">Tests for ampicillin resistance given by protein added to medium</a></li> | ||
+ | |||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=6_August_2008&arg1=7_August_2008&arg2=8_August_2008&arg3=11_August_2008&name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29">Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=6_August_2008&arg1=7_August_2008&arg2=8_August_2008&arg3=11_August_2008&name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29>Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10) </a></li> | ||
+ | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=15_September_2008&arg1=16_September_2008&arg2=17_September_2008&arg3=18_September_2008&arg4=19_September_2008&arg5=22_September_2008&name=%27Hunter%20and%20prey%27%20system%20tests%3A%20Competition%20tests">'Hunter and prey' system tests: Competition tests</a></li> | ||
+ | |||
+ | |||
+ | |||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | <h3><a OnClick=switchMenu('bricks')>Preparation of BioBricks</a></h3> | ||
+ | <div name="menu_item" id="bricks" style="display: none"> | ||
+ | |||
+ | <ol> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=17_September_2008&arg1=18_September_2008&name=Optimisation%20of%20primers%20for%20preparation%20of%20parts">Optimisation of primers for preparation of parts</a></li> | ||
+ | |||
+ | <li class="nb_toc1"><a OnClick=switchMenu('bricks-vect','submenu_item')>Preparation of vectors for BioBricks parts cloning</a></li> | ||
+ | <div name="submenu_item" id="bricks-vect" style="display: none"> | ||
+ | <ul> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=3_October_2008&arg1=6_October_2008&arg2=7_October_2008&arg3=8_October_2008&arg4=9_October_2008&name=Preparation%20of%20vector%20for%20pT7%20constructs">Preparation of vector for pT7 constructs</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=29_September_2008&arg1=30_September_2008&arg2=1_October_2008&name=Preparation%20of%20vectors%20for%20Biobricks">Preparation of vectors for Biobricks</a></li> | ||
+ | |||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | <li class="nb_toc1"><a OnClick=switchMenu('bricks-coding','submenu_item')>Preparation of BioBricks parts (coding type)</a></li> | ||
+ | <div name="submenu_item" id="bricks-coding" style="display: none"> | ||
+ | <ul> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=29_September_2008&arg1=30_September_2008&arg2=1_October_2008&arg3=2_October_2008&name=Preparation%20of%20Z%28BBa_K103004%29">Preparation of Z(BBa_K103004)</a></li> | ||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=1_October_2008&arg1=6_October_2008&arg2=7_October_2008&arg3=8_October_2008&arg4=9_October_2008&name=Preparation%20of%20AID%28BBa_K103001%29">Preparation of AID(BBa_K103001)</a></li> | ||
+ | |||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=3&arg0=20_October_2008&arg1=21_October_2008&arg2=22_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20%28BBa_K103014%29">Preparation of OmpA_linker_alpha_linker (BBa_K103014)</a></li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29">Preparation of ΔA (BBa_K103003)</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=10&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&arg6=13_October_2008&arg7=14_October_2008&arg8=15_October_2008&arg9=16_October_2008&name=Preparation%20of%20linker_alpha%20%28BBa_K103009%29">Preparation of linker_alpha (BBa_K103009)</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&name=Preparation%20of%20linker_omega%20%28BBa_K103013%29">Preparation of linker_omega (BBa_K103013)</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=15&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&arg5=6_October_2008&arg6=7_October_2008&arg7=8_October_2008&arg8=13_October_2008&arg9=14_October_2008&arg10=15_October_2008&arg11=16_October_2008&arg12=17_October_2008&arg13=21_October_2008&arg14=22_October_2008&name=Preparation%20of%20OmpA-linker-omega-linker%20%28BBa_K103016%29">Preparation of OmpA-linker-omega-linker (BBa_K103016)</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=18_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=2_October_2008&arg4=3_October_2008&name=Preparation%20of%20OmpA-linker%20%28BBa_K103006%29">Preparation of OmpA-linker (BBa_K103006)</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | <li class="nb_toc1"><a OnClick=switchMenu('bricks-generator','submenu_item')>Preparation of BioBricks parts (coding type)</a></li> | ||
+ | <div name="submenu_item" id="bricks-generator" style="display: none"> | ||
+ | <ul> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=5&arg0=10_October_2008&arg1=13_October_2008&arg2=14_October_2008&arg3=15_October_2008&arg4=16_October_2008&name=Preparation%20of%20OmpA_linker_alpha_linker%20under%20Plac%20%28BBa_K103017%29">Preparation of OmpA_linker_alpha_linker under Plac (BBa_K103017)</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_September_2008&arg1=30_September_2008&arg2=1_October_2008&arg3=2_October_2008&arg4=6_October_2008&arg5=7_October_2008&arg6=8_October_2008&arg7=9_October_2008&name=Preparation%20of%20OmpA_linker_omega_linker%20under%20Plac%20%28BBa_K103018%29">Preparation of OmpA_linker_omega_linker under Plac (BBa_K103018)</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=25_September_2008&arg1=9_October_2008&arg2=10_October_2008&arg3=13_October_2008&arg4=14_October_2008&arg5=16_October_2008&arg6=17_October_2008&arg7=20_October_2008&arg8=21_October_2008&name=Preparation%20of%20alpha_linker%20under%20PT7%20%28BBa_K103019%29">Preparation of alpha_linker under PT7 (BBa_K103019)</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=25_September_2008&arg1=29_September_2008&arg2=30_September_2008&arg3=9_October_2008&arg4=10_October_2008&arg5=13_October_2008&arg6=14_October_2008&name=Preparation%20of%20omega_linker%20under%20PT7%20%28BBa_K103020%29">Preparation of omega_linker under PT7 (BBa_K103020)</a></li> | ||
+ | |||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=12&arg0=7_October_2008&arg1=8_October_2008&arg2=9_October_2008&arg3=10_October_2008&arg4=13_October_2008&arg5=14_October_2008&arg6=15_October_2008&arg7=16_October_2008&arg8=17_October_2008&arg9=20_October_2008&arg10=21_October_2008&arg11=22_October_2008&name=Preparation%20of%20AID%20under%20pBAD/araC%20%28BBa_K103002%29">Preparation of AID under pBAD/araC (BBa_K103002)</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | </ol> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | <h3><a OnClick=switchMenu('other')>Other activities</a></h3> | ||
+ | <div name="menu_item" id="other" style="display: none"> | ||
+ | <ol> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=6_September_2008&arg1=14_September_2008&name=Wiki%20scripting">Wiki scripting</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=8_September_2008&name=Our%20webpage%20has%20a%20completely%20new%20layout">Our webpage has a completely new layout</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=11_September_2008&name=We%27re%20fighting%20hard%20to%20obtain%20funds%20for%20the%20visit%20on%20iGEM2008%20Jamboree%21">We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=23_October_2008&name=Sending%20our%20bricks%20to%20the%20Registry">Sending our bricks to the Registry</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=24_October_2008&name=Computer%20meeting%20to%20finish%20our%20webpage">Computer meeting to finish our webpage</a></li> | ||
+ | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=1&arg0=10_October_2008&name=Visit%20in%20US%20Embassy">Visit in US Embassy</a></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </td><td valign="top"> </html> | ||
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+ | |- | ||
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+ | |- | ||
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| | | | ||
|}<html> | |}<html> | ||
+ | </td></tr></table> | ||
<h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2> | <h2 align="left"><a href=https://2008.igem.org/Wiki/Team:Warsaw/protocols_list>Protocols</a></h2> | ||
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Revision as of 00:46, 29 October 2008
ProtocolsPrimersVectorsStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |