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Infection
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(New page: ==Infection Protocol== Count cells. Add 1x105 cells to each well. Add 0.8 ul polybrene for each ml of media in the well. Slide to mix. Add virus – one tube (~15ul) to each well. (B...)
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(New page: ==Infection Protocol== Count cells. Add 1x105 cells to each well. Add 0.8 ul polybrene for each ml of media in the well. Slide to mix. Add virus – one tube (~15ul) to each well. (B...)
Newer edit →
Revision as of 01:58, 29 October 2008
Infection Protocol
Count cells.
Add 1x105 cells to each well.
Add 0.8 ul polybrene for each ml of media in the well.
Slide to mix.
Add virus – one tube (~15ul) to each well. (BLEACH everything used this point and later!)
Incubate 8-24 hours.
Change media (1mL per well).
