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Restriction Map/ Restriction Digest
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==Restriction Map Design== | ==Restriction Map Design== | ||
| - | Restriction maps are | + | Restriction maps are designed through Vector NTI plasmid sequences. Restriction sites with compatible enzymes (similar temperatures and buffers used) are found, and those enzymes are then used to digest the plasmid. The restriction site must also be in the proper area of the plasmid for insertion of other necessary isolated DNA through the process of ligation. |
==Restriction Digest Protocol== | ==Restriction Digest Protocol== | ||
Revision as of 02:13, 29 October 2008
Restriction Map Design
Restriction maps are designed through Vector NTI plasmid sequences. Restriction sites with compatible enzymes (similar temperatures and buffers used) are found, and those enzymes are then used to digest the plasmid. The restriction site must also be in the proper area of the plasmid for insertion of other necessary isolated DNA through the process of ligation.
