Wisconsin:Lignin Project/29 August 2008

From 2008.igem.org

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Second His-tag purification resulted again with no protein.  Now it is believed to be too small an amount for the nanodrop to measure.
Second His-tag purification resulted again with no protein.  Now it is believed to be too small an amount for the nanodrop to measure.
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'''Team Fungus:'''<br>
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Remade assay solutions due to contaminants.<br>
 +
Re-ran Azure B assay on 96 well plate.<br>
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Latest revision as of 02:45, 29 October 2008

Igemwibanner.gif
Team Sorbitol:

Performed PCR cleanup and reactions for sequencing.
Submitted for sequencing.
Sequencing results received same day. All colonies perfect matches with new primers. Success!
Ligation product transformed into E.coli.
Second His-tag purification resulted again with no protein. Now it is believed to be too small an amount for the nanodrop to measure.

Team Fungus:
Remade assay solutions due to contaminants.
Re-ran Azure B assay on 96 well plate.

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