Team:The University of Alberta/26 October 2008

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Revision as of 02:53, 29 October 2008

Jason

I performed a mini-prep of pUC57-ER, subsequently digesting it with XbaI and PstI, gel purifying the fragment and using it one of the following ligations.

I set up ligations for of pSB103+ER, pSB103+laqIQ-ERE-TetR-pTET-RFP, pSB103+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB and left them overnight at 16 degrees C.

i was awesome today.

David

yesterday's transformations didn't work. not at all. no colonies. what is up with that?

Revision as of 02:53, 29 October 2008 (view source) Beardarm (Talk \| contribs) (→Jason) ← Older edit		Revision as of 02:53, 29 October 2008 (view source) Beardarm (Talk \| contribs) (→David) Newer edit →
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-	~~yesterdays~~ transformations didn't work. not at all. no colonies. what is up with that?	+	yesterday's transformations didn't work. not at all. no colonies. what is up with that?