Team:The University of Alberta/24 October 2008

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(New page: ==David== i digested laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI, pTET-BisdA-pTET-BisdB with XbaI+PstI, pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP....)
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Revision as of 02:57, 29 October 2008

David

i digested laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI, 

          pTET-BisdA-pTET-BisdB with XbaI+PstI,
          pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.

i gel purified the desired products.

i set up ligations for pSB103+laqIQ-ERE-TetR-pTET-RFP, pSB103+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.

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