Wiki/Team:Warsaw/igem notebook.htm
From 2008.igem.org
(Difference between revisions)
Line 165: | Line 165: | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=6_August_2008&arg1=7_August_2008&arg2=8_August_2008&arg3=11_August_2008&name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29">Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=6_August_2008&arg1=7_August_2008&arg2=8_August_2008&arg3=11_August_2008&name=Checking%20whether%20degradation%20of%20the%20fusions%20with%20OmpA%20is%20caused%20by%20Lon%20and%20OmpT%20proteases%20%28present%20in%20TOP10%29">Checking whether degradation of the fusions with OmpA is caused by Lon and OmpT proteases (present in TOP10)</a></li> | ||
- | |||
<li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li> | <li class="nb_toc"><a href=https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=2&arg0=12_August_2008&arg1=13_August_2008&name=Checking%20for%20presence%20of%20protein%20A%20on%20the%20cell%20membrane>Checking for presence of protein A on the cell membrane </a></li> | ||
<li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=15_September_2008&arg1=16_September_2008&arg2=17_September_2008&arg3=18_September_2008&arg4=19_September_2008&arg5=22_September_2008&name=%27Hunter%20and%20prey%27%20system%20tests%3A%20Competition%20tests">'Hunter and prey' system tests: Competition tests</a></li> | <li class="nb_toc"><a href="https://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=15_September_2008&arg1=16_September_2008&arg2=17_September_2008&arg3=18_September_2008&arg4=19_September_2008&arg5=22_September_2008&name=%27Hunter%20and%20prey%27%20system%20tests%3A%20Competition%20tests">'Hunter and prey' system tests: Competition tests</a></li> |
Revision as of 02:57, 29 October 2008
ProtocolsPrimersVectorsStrainsRosetta(DE3)pLysSF- ompT hsdSB(RB- mB-) gal dcm λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) pLysSRARE (CamR) TOP10 (Invitrogen)F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- NR9458 (IBB collection; in notebook called mutD5)ara thi Δ pro lac mutD5 zaf-13 ::Tn10 GM2163ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |