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Team:The University of Alberta/24 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==David== i digested laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI, pTET-BisdA-pTET-BisdB with XbaI+PstI, pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP....) |
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| - | i digested laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI, | + | i digested |
| + | laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI, | ||
pTET-BisdA-pTET-BisdB with XbaI+PstI, | pTET-BisdA-pTET-BisdB with XbaI+PstI, | ||
pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP. | pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP. | ||
Revision as of 02:57, 29 October 2008
David
i digested
laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI,
pTET-BisdA-pTET-BisdB with XbaI+PstI,
pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.
i gel purified the desired products.
i set up ligations for pSB103+laqIQ-ERE-TetR-pTET-RFP, pSB103+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.
