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Team:The University of Alberta/6 August 2008
From 2008.igem.org
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*Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday. | *Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday. | ||
*Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b. | *Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b. | ||
| + | ''' Jason''' | ||
| + | * overnight of transformants (MCS+CanR, LacIQERE+TETR) | ||
| + | *transforming ligations | ||
| + | cam35sr +PR and CAMR and MCS | ||
Latest revision as of 03:18, 29 October 2008
Chris
- Got some strange results from the PCR I did yesterday. There was a band in the digested sample BUT it wasnt the size of GFP; rather it was ~1500bp, the expected size for Tryp. Even odder is that the same band is in the uncut sample as well, in addition to the expected GFP sized band. I discussed this with Doug for quite some time and we were unable to come to a conclusion about what's going on with Tryp. I figure tomorrow I'll just go back to the drawing board and start over...
Saima
- Mini-preped the O/N's of Col. 4,5,& 6 of I0500+ER01 and I0500+ER02. Can be found in Mini-prep Box 3.
- Sequenced Minipreps of Col.4 & Col.5 of I0500+ER01, and Col.4 of I0500+ER02. To be taken to MBSU on Thrursday. Results to be back by Friday.
- Set up the O/N's for I0500+ER01 col.6, I0500+ER02 col.4, I0500 in new vector, GFP+B0034 Col.1a & 1b.
Jason
- overnight of transformants (MCS+CanR, LacIQERE+TETR)
- transforming ligations
cam35sr +PR and CAMR and MCS
