Wisconsin: Lignin Project/8 July 2008

From 2008.igem.org

(Difference between revisions)
 
Line 8: Line 8:
Used this DNA in a PCR sequencing reaction.<br>
Used this DNA in a PCR sequencing reaction.<br>
The product was wrapped in foil (to keep out the light) and stored at 4C overnight.<br>
The product was wrapped in foil (to keep out the light) and stored at 4C overnight.<br>
 +
'''Team Fungus:'''<br>
 +
Ran PCR3 product out on 1% agarose gel. Ran side by side gels to test gel boxes.<br>
 +
Sequencing primers arrived.<br>
 +
Purified PCR product from PCR2 from 7/7.<br>
 +
Purified double digested insert from 7/3.<br>
 +
Made LB+Amp plates.<br>
 +
Received lignin peroxidase from Sigma.<br>
 +
Purified pET28a from overnight cultures.<br>
 +
Purified PCR product form 7/7 PCR3.<br>
 +
Ran PCR3 out on 1% agarose gel.<br>
 +
Isolated genomic DNA from BL21.<br>
 +
Purified PCR product form 7/7 PCR2.<br>
 +
Ligated purified PCR2 product into PGEM-T easy.<br>
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Latest revision as of 03:21, 29 October 2008

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Team Sorbitol:

Harvested the DNA from the overnight colonies.
Used this DNA in a PCR sequencing reaction.
The product was wrapped in foil (to keep out the light) and stored at 4C overnight.
Team Fungus:
Ran PCR3 product out on 1% agarose gel. Ran side by side gels to test gel boxes.
Sequencing primers arrived.
Purified PCR product from PCR2 from 7/7.
Purified double digested insert from 7/3.
Made LB+Amp plates.
Received lignin peroxidase from Sigma.
Purified pET28a from overnight cultures.
Purified PCR product form 7/7 PCR3.
Ran PCR3 out on 1% agarose gel.
Isolated genomic DNA from BL21.
Purified PCR product form 7/7 PCR2.
Ligated purified PCR2 product into PGEM-T easy.

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