Team:The University of Alberta/24 October 2008

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Revision as of 03:21, 29 October 2008

David

i digested

          laqIQ-ERE-TetR-pTET-RFP with XbaI+PstI,
          pTET-BisdA-pTET-BisdB with XbaI+PstI,
          pUC57-laqIQ-ERE-TetR-pTET-RFP with SphI+PstI and treated with CIP.

i gel purified the desired products.

i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.

Revision as of 02:57, 29 October 2008 (view source) Beardarm (Talk \| contribs) (→David) ← Older edit		Revision as of 03:21, 29 October 2008 (view source) Beardarm (Talk \| contribs) (→David) Newer edit →
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	i gel purified the desired products.		i gel purified the desired products.

-	i set up ligations for ~~pSB103~~+laqIQ-ERE-TetR-pTET-RFP, ~~pSB103~~+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.	+	i set up ligations for pSB1A3+laqIQ-ERE-TetR-pTET-RFP, pSB1A3+pTET-BisdA-pTET-BisdB, pUC57-laqIQ-ERE-TetR-pTET-RFP+pTET-BisdA-pTET-BisdB overnight at 16 degrees C.