Wisconsin: Lignin Project/10 July 2008

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(Difference between revisions)

Latest revision as of 03:28, 29 October 2008

Team Sorbitol:

Got the sequencing data back and found that colony 2 was correct and will be used for all future experiments.
Started the following cultures:
DH5a-PBAD30 : to harvest the plasmid to clone out the srl operon
DH5a-pBAD30-srlD: from colony 2 to freeze down for back up
DH5a-pBAD18 - to insert srld and the operon into MG1655, JW3890, RL257
This was done to make chemically competent
Team Fungus:
Ran out gradient PCR products on 1% agarose gel. No bands.
Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template. Ran PCR out on gel. Only positive control showed up.
Made LB+Amp+XGal plates.
Attempted transformation again.

@@ Line 12: / Line 12: @@
 This was done to make chemically competent<br>
 '''Team Fungus:'''<br>
-Ran TAQ PCR again in one more tube due to low amplification results using only 1 uL of cDNA template.
+Ran out gradient PCR products on 1% agarose gel. No bands.<br>
+Ran TAQ gradient PCR again in one more tube due to low amplification results using only 1 uL of cDNA template.
+Ran PCR out on gel. Only positive control showed up.<br>
+Made LB+Amp+XGal plates.<br>
+Attempted transformation again.<br>
 |}