Wisconsin: Lignin Project/16 July 2008

From 2008.igem.org

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Line 12: Line 12:
'''Team Fungus'''<br>
'''Team Fungus'''<br>
Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).<br>
Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).<br>
 +
Started overnight cultures from transformation.<br>
 +
Ran touchdown PCR using Taq using "good stuff" as template (correct band from 7/11).<br>
 +
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|}

Latest revision as of 03:39, 29 October 2008

Igemwibanner.gif
Team Sorbitol

Growth curves directly comparing Wt+(MG1655) and tpiA knockout in C-media.
Start PCR srl operon from genomic DNA provided by Dr. Pfleger instead of MG1655. Still no band is shown on the gel.


Team Fungus
Ran gradient PCR reaction using TAQ and "Good Stuff" template dilute (1:10).
Started overnight cultures from transformation.
Ran touchdown PCR using Taq using "good stuff" as template (correct band from 7/11).

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