Team:UCSF/Parts

From 2008.igem.org

(Difference between revisions)
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'''Project-relevant AarI TOPO clones'''  
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'''Project-relevant AarI Parts'''  
''digestion of these plasmids with AarI will release a fragment for AarI cloning.''
''digestion of these plasmids with AarI will release a fragment for AarI cloning.''
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*Sir4 AD
*Sir4 AD
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 +
'''AarI acceptor vectors (empty)'''
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''These accept AarI digested parts, and include a promoter/terminator.''
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 +
 +
 +
'''AarI Shuttle Vector'''
 +
''So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.
 +
 +
*AarI--Biobrick Shuttle AB
 +
 +
*AarI--Biobrick Shuttle BD
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 +
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'''Bonus AarI Parts'''
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''These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.''
 +
 +
Esa1 AB
GFP BD
GFP BD
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Sas2 BD
Sas2 BD
-
 
-
'''AarI acceptor vectors (empty)'''
 
-
''These accept AarI digested parts, and include a promoter/terminator.''
 
-
 
-
'''AarI Shuttle Vector'''
 
-
''So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.
 
-
 
-
*AarI--Biobrick Shuttle AB
 
-
 
-
*AarI--Biobrick Shuttle BD
 
-
 
-
 
-
'''Bonus AarI Parts'''
 
-
''These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.''
 
-
 
-
Esa1 AB
 
-
 
-
 

Revision as of 04:44, 29 October 2008

For complete details and sequence, see the Registry: http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=UCSF&Done=1


Project-relevant AarI Parts digestion of these plasmids with AarI will release a fragment for AarI cloning.

  • LexA (1-87 a.a) AB
  • Sir2 BD
  • GFP AD
  • Ssn8 AD
  • Sir3 A!D
  • Sir4 AD

AarI acceptor vectors (empty) These accept AarI digested parts, and include a promoter/terminator.


AarI Shuttle Vector So you don't want to use AarI cloning? OK. We've provided a shuttle vector allows one-sublcone conversion of AarI parts into biobricks.

  • AarI--Biobrick Shuttle AB
  • AarI--Biobrick Shuttle BD


Bonus AarI Parts These are provided to the registry to facilitate getting started with AarI cloning. Some we had planned to use, but didn't get around to, and others are selected from the lab database of AarI parts.

Esa1 AB

GFP BD

Sir4

Sir2 AB


mCherry AB

mCherry BD


LexA (1-87) BD

Pif3 AB

PhyB AB

Sas2 AB

Sas2 BD



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