Team:iHKU/biobrick

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                       <th width="10%">&nbsp;</th>
                       <th width="10%">&nbsp;</th>
                         <td width="80%" align="left"><h1 class="style7">Biobrick</h1>
                         <td width="80%" align="left"><h1 class="style7">Biobrick</h1>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/7/7d/Biobrick_pic1.png" width="155" height="51" /></p>
 
-
                          <p>cheZ: This part contains the cheZ operon  coding region without an rbs nor terminator. cheZ protein is responsible for  the dephosphorylation of cheY protein in bacteria flagella movement.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/1/1d/Biobrick_pic2.png" width="143" height="59" /></p>
+
                           <p>BBa_K094100 <img src="https://static.igem.org/mediawiki/2008/6/63/Biobrick_pic1.gif" width="50" height="52" /><img width="50" height="52" src="biobrick_clip_image002.jpg" /><br />
-
                          <p>cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein.  This part contains the coding region only.</p>
+
cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/5/51/Biobrick_pic3.png" width="175" height="47" /></p>
 
-
                          <p>pTetR-rbs-cheZ: This part uses BBa_J61003  as a backbone and cheZ as the inserted gene. cheZ protein is responsible for  the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ  operon lacks a terminator.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/c/cf/Biobrick_pic4.png" width="176" height="55" /></p>
+
                           <p>BBa_K094101  <img src="https://static.igem.org/mediawiki/2008/b/bb/Biobrick_pic2.gif" width="146" height="55" /><img width="146" height="55" src="biobrick_clip_image002_0003.jpg" /><br />
-
                          <p>pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY  protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ  protein. This part lacks a terminator.</p>
+
plambda(R-O12)-rbs-cheZ: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein.  cheZ protein is responsible for the dephosphorylation of cheY protein in  bacteria flagella movement. This part lacks a terminator.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/d/db/Biobrick_pic5.png" width="172" height="61" /></p>
 
-
                          <p>pTetR-rbs-lacZ alpha: This is a composite  part by putting lacZ alpha fragment and J61003 together. This part lacks a  terminator.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/8/82/Biobrick_pic6.png" width="143" height="50" /></p>
+
                           <p>BBa_K094102 <img src="image/biobrick_pic3.JPG" width="175" height="47" /><img width="122" height="54" src="biobrick_clip_image002_0004.jpg" /><br />
-
                          <p>pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.</p>
+
pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for  the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ  operon lacks a terminator.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/c/ce/Biobrick_pic7.png" width="245" height="68" /></p>
 
-
                          <p>pLacIq-rbs-LacI-terminator: This LacI gene  contains a mutated pLacIq promoter which enables high level of transcription.  This ensures effective repression of pLacI promoter that might be present.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/2/2b/Biobrick_pic8.png" width="251" height="63" /></p>
+
                           <p>BBa_K094103  <img src="image/biobrick_pic4.JPG" width="176" height="55" /><img width="156" height="56" src="biobrick_clip_image002_0005.jpg" /><br />
-
                          <p>Rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.</p>
+
plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein  is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/1/1b/Biobrick_pic9.png" width="275" height="67" /></p>
 
-
                          <p>pLacI/ara-1-rbs-luxI-luxR-terminator: This  is a quorum sensing composition. Induced by IPTG or arabinose, this part can  establish a quorum sensing system, with the luxI protein producing AHL and luxR  protein as the AHL density sensor.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/4/4c/Biobrick_pic10.png" width="214" height="63" /></p>
+
                           <p>BBa_K094104  <img src="image/biobrick_pic5.JPG" width="172" height="61" /><img width="227" height="48" src="biobrick_clip_image002_0006.jpg" /><br />
-
                          <p>plambda(R-O12)-rbs-cheZ: This part contains  a lambda promoter that is constitutively on and can be repressed by CI protein.  cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.</p>
+
pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/7/72/Biobrick_pic11.png" width="364" height="68" /></p>
+
                           <p>&nbsp;</p>
 +
                          <p>BBa_K094105 <img src="image/biobrick_pic6.JPG" width="143" height="50" /><img width="292" height="58" src="biobrick_clip_image002_0007.jpg" /><br />
 +
plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double  terminator: This part can be used to measure the gene expression of cheZ under  the control of promoter plambda(R-O12).</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094106  <img src="image/biobrick_pic7.JPG" width="245" height="68" /><img width="277" height="77" src="biobrick_clip_image002_0008.jpg" /><br />
 +
Plux-rbs-CI-terminator-plambda-rbs-cheZ:  This part encodes a CI protein that is used to repress lambda promoter. The  part is controlled by the lux promoter that can be induced by luxR protein  under high AHL concentration. In this part, the expression of cheZ is repressed  by increasing AHL concentration in the environment in the presence of luxR  protein. In other circumstances, the cheZ gene express constitutively.</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094110 <img src="image/biobrick_pic8.JPG" width="251" height="63" /><img width="51" height="49" src="biobrick_clip_image002_0009.jpg" /><br />
 +
cheY*: This is the gene coding for cheY  protein which controls flagella movement. This sequence, however, is mutated to  give a mutant cheY protein which will not respond to the presence of cheZ  protein. This part contains the coding region only.</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094111<img src="image/biobrick_pic9.JPG" width="275" height="67" /><img width="122" height="53" src="biobrick_clip_image002_0010.jpg" /><br />
 +
pTetR-rbs-cheY*: This part uses BBa_J61003  as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY  protein which controls flagella movement. This sequence, however, is mutated to  give a mutant cheY protein which will not respond to the presence of cheZ  protein. This part lacks a terminator.</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094112  <img src="image/biobrick_pic10.JPG" width="214" height="63" /><img width="150" height="58" src="biobrick_clip_image002_0011.jpg" /><br />
 +
luxR-rbs-luxI-terminator: : The bacterial <a href="http://en.wikipedia.org/wiki/Luciferin" title="Luciferin">luciferin</a>-<a href="http://en.wikipedia.org/wiki/Luciferase" title="Luciferase">luciferase</a> system is encoded by a set of genes labelled the <a href="http://en.wikipedia.org/w/index.php?title=Lux_operon&amp;action=edit&amp;redlink=1" title="Lux operon (page does not exist)">Lux operon</a>. In <em>V. fischeri</em> five such genes (<em>LuxCDABE</em>) have been identified as active in the  emission of visible light, and two genes (<em>LuxR</em> and <em>LuxI</em>) are  involved in regulating the <a href="http://en.wikipedia.org/wiki/Operon" title="Operon">operon</a>. This  part can be utilized as a part of quorum sensing system.</p>
 +
                          <p>&nbsp;</p>
 +
                          <p><img src="image/biobrick_pic11.JPG" width="364" height="68" /></p>
                           <p>Plux-rbs-CI-terminator-plambda-rbs-cheZ:  This is a part in which the expression of cheZ is repressed by increasing AHL  concentration in the environment in the presence of luxR protein. In other  circumstances the cheZ gene is constitutively expressed.</p>
                           <p>Plux-rbs-CI-terminator-plambda-rbs-cheZ:  This is a part in which the expression of cheZ is repressed by increasing AHL  concentration in the environment in the presence of luxR protein. In other  circumstances the cheZ gene is constitutively expressed.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/0/09/Biobrick_pic12.png" width="243" height="65" /></p>
 
-
                          <p>plux-rbs-cheZ-terminator: This is a cheZ  gene that can be induced by luxR protein under high AHL concentration. cheZ  protein is responsible for the dephosphorylation of cheY protein in bacteria  flagella movement.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/d/d1/Biobrick_pic13.png" width="240" height="64" /></p>
+
                           <p>BBa_K094113<img src="image/biobrick_pic12.JPG" width="243" height="65" /><img width="50" height="47" src="biobrick_clip_image002_0012.jpg" /><br />
-
                           <p>plux-rbs-CI-terminator: This part encodes a  CI protein that is used to repress lambda promoter. The part is controlled by a lux promoter that can be induced by luxR protein under high AHL concentration.</p>
+
lacZ: This is a reporter gene that codes  for beta galactosidase.</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094120 <img src="image/biobrick_pic13.JPG" width="240" height="64" /><img width="57" height="61" src="biobrick_clip_image002_0013.jpg" /><br />
 +
pLacI/ara-1: This promoter can be repressed  by lacI protein and induced by either IPTG or arabinose.</p>
 +
                           <p>&nbsp;</p>
 +
                          <p>&nbsp;</p>
 +
                          <p>BBa_K094130  <img src="image/biobrick_pic14.JPG" width="235" height="59" /><img width="164" height="64" src="biobrick_clip_image002_0014.jpg" /><br />
 +
rbs-mRFP1-terminator: This part is adapted  from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/8/8d/Biobrick_pic14.png" width="235" height="59" /></p>
 
-
                          <p>pLacI/ara-1-rbs-luxI-terminator: A luxI  operon controlled by a LacI/ara-1 promoter.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="https://static.igem.org/mediawiki/2008/2/25/Biobrick_pic15.png" width="215" height="64" /></p>
+
                           <p>BBa_K094141 <img src="image/biobrick_pic15.JPG" width="215" height="64" /><img width="171" height="59" src="biobrick_clip_image002_0015.jpg" /><br />
-
                          <p>plambda(R-O12)-rbs-lacZ: A lacZ gene with a lambda(R-O12) promoter. This part is constitutively expressed but repressed in the presence of CI protein. This part lacks a terminator.</p>
+
pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI  promoter that might be present. </p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="https://static.igem.org/mediawiki/2008/0/01/Biobrick_pic16.png" width="418" height="67" /></p>
 
-
                          <p>pLacIq-rbs-lacI-terminator-pLacI-rbs-mRFP1-terminator:  This part is composited with a lacI operon and an mRFP operon. The LacI operon  contains a mutated lacIq promoter that enables high level of transcription. The  mRFP operon is spontaneously repressed by the LacI operon, and can be turned on  by inducing with IPTG.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="image/biobrick_pic17.JPG" width="306" height="65" /></p>
+
                           <p>BBa_K094150  <img src="image/biobrick_pic16.JPG" width="418" height="67" /><img width="227" height="62" src="biobrick_clip_image002_0016.jpg" /><br />
-
                          <p>cheZ-pLacI-rbs-mRFP1-terminator: the cheZ gene is installed in front of mRFP operon. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This cheZ gene  is without a promoter, rbs or terminator.</p>
+
pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR  protein as the AHL density sensor.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                          <p><img src="image/biobrick_pic18.JPG" width="310" height="68" /></p>
 
-
                          <p>cheY*-pLacI-rbs-mRFP1-terminator: the cheY*  gene is installed in front of mRFP operon. cheY is the gene coding for cheY  protein which controls flagella movement. This sequence, however, is mutated to  give a mutant cheY protein which will not respond to the presence of cheZ  protein.? This cheY* gene is without a  promoter, rbs or terminator.</p>
 
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="image/biobrick_pic19.JPG" width="277" height="65" /></p>
+
                           <p>BBa_K094151  <img src="image/biobrick_pic17.JPG" width="306" height="65" /><img width="162" height="61" src="biobrick_clip_image002_0017.jpg" /><br />
-
                          <p>cheZ-pLacIq-rbs-lacI-terminator: the cheZ gene is installed in front of a lacI operon. cheZ protein is responsible for  the dephosphorylation of cheY protein in bacteria flagella movement. This lacI  operon contains a mutated LacIq promoter which enables high level of  transcription. The cheZ gene is without a promoter, rbs or terminator.</p>
+
pLacI/ara-1-rbs-luxI-terminator: The luxI operon is controlled by promoter LacI/ara-1.</p>
                           <p>&nbsp;</p>
                           <p>&nbsp;</p>
-
                           <p><img src="image/biobrick_pic20.JPG" width="277" height="62" /></p>
+
                           </td>
-
                          <p>cheY*-pLacIq-rbs-lacI-terminator:  the cheY* gene is installed in front of a lacI operon. cheY is the gene coding  for cheY protein which controls flagella movement. This sequence, however, is  mutated to give a mutant cheY protein which will not respond to the presence of  cheZ protein. This lacI operon contains a mutated LacIq promoter which enables  high level of transcription. The cheY* gene is without a promoter, rbs</p></td>
+
                         <td width="10%">&nbsp;</td>
                         <td width="10%">&nbsp;</td>
                       </tr>
                       </tr>

Revision as of 09:17, 29 October 2008

 

Biobrick

 

BBa_K094100
cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

 

 

BBa_K094101 
plambda(R-O12)-rbs-cheZ: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.

 

 

BBa_K094102
pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.

 

 

BBa_K094103  
plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.

 

 

BBa_K094104  
pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.

 

 

BBa_K094105
plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double terminator: This part can be used to measure the gene expression of cheZ under the control of promoter plambda(R-O12).

 

 

BBa_K094106 
Plux-rbs-CI-terminator-plambda-rbs-cheZ: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by the lux promoter that can be induced by luxR protein under high AHL concentration. In this part, the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances, the cheZ gene express constitutively.

 

BBa_K094110
cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.

 

 

BBa_K094111
pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.

 

 

BBa_K094112  
luxR-rbs-luxI-terminator: : The bacterial luciferin-luciferase system is encoded by a set of genes labelled the Lux operon. In V. fischeri five such genes (LuxCDABE) have been identified as active in the emission of visible light, and two genes (LuxR and LuxI) are involved in regulating the operon. This part can be utilized as a part of quorum sensing system.

 

Plux-rbs-CI-terminator-plambda-rbs-cheZ: This is a part in which the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances the cheZ gene is constitutively expressed.

 

 

BBa_K094113
lacZ: This is a reporter gene that codes for beta galactosidase.

 

 

BBa_K094120
pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.

 

 

BBa_K094130  
rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.

 

 

BBa_K094141
pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present.

 

 

BBa_K094150 
pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.

 

 

BBa_K094151  
pLacI/ara-1-rbs-luxI-terminator: The luxI operon is controlled by promoter LacI/ara-1.

 

  

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