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Team:Rice University/Notebook/21 May 2008
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*Taylor Stevenson | *Taylor Stevenson | ||
**Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells. | **Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells. | ||
| - | *#Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min | + | *#Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min. |
*#Electropulse parameters: 1.8 kV & 5.4 ms | *#Electropulse parameters: 1.8 kV & 5.4 ms | ||
| - | *# | + | *#Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol. Plates were cultured O/N. |
**'''Result'''-no colonies were observed at any dilution | **'''Result'''-no colonies were observed at any dilution | ||
Latest revision as of 17:15, 25 June 2008
Wednesday 21 May
- Taylor Stevenson
- Transformation From New Registry (Paper Punches)-Electroporation of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells.
- Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min.
- Electropulse parameters: 1.8 kV & 5.4 ms
- Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol. Plates were cultured O/N.
- Result-no colonies were observed at any dilution
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